Title: Contribution of secretory leukocyte proteinase inhibitor to the antiprotease defense system of the peripheral lung: effect of ozone-induced acute inflammation.
Authors: Nadziejko, C; Finkelstein, I; Balmes, J R
Published In Am J Respir Crit Care Med, (1995 Nov)
Abstract: Secretory leukocyte protease inhibitor (SLPI) and elafin are structurally similar, low-molecular-weight antiproteases produced in the lung. We have developed a simple method for distinguishing the antiprotease activities of SLPI and elafin in lung lavage fluid from those of alpha 1-antitrypsin (alpha 1-AT) that is based on the resistance of the low-molecular-weight antiproteases to inactivation by cetyltrimethylammonium bromide. In a study of 23 healthy, nonsmoking volunteers, we found that the low-molecular-weight antiproteases accounted for 22 +/- 2% (mean +/- SEM, n = 23) of the total neutrophil elastase-inhibitory capacity of human bronchoalveolar lavage fluid (BALF). Elafin activity was below the limit of detection. SLPI activity (as measured by inhibition of alpha-chymotrypsin) accounted for 72 +/- 4% (mean +/- SEM, n = 23) of the low-molecular-weight antiprotease activity in BALF. Measurements of SLPI in the lavage fluid samples by enzyme-linked immunosorbent assay (ELISA) agreed closely with values obtained by measuring the activity of this inhibitor. The activity of the low-molecular-weight antiproteases decreased significantly (p < 0.05), from 9.0 +/- 0.8 to 7.0 +/- 0.6 pmol of neutrophil elastase inhibited per mL (mean +/- SEM, n = 23), following acute ozone exposure.
PubMed ID: 7582300
MeSH Terms: Acute Disease; Adult; Bronchoalveolar Lavage Fluid/chemistry; Bronchoscopy; Cross-Over Studies; Enzyme-Linked Immunosorbent Assay; Female; Humans; Lung/drug effects; Lung/enzymology*; Male; Molecular Weight; Ozone/adverse effects*; Pneumonia/chemically induced; Pneumonia/enzymology*; Proteins/analysis; Proteins/physiology*; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Serine Proteinase Inhibitors/analysis; Serine Proteinase Inhibitors/physiology*; Single-Blind Method