Title: Cybrid technology.
Authors: Bacman, Sandra R; Nissanka, Nadee; Moraes, Carlos T
Published In Methods Cell Biol, (2020)
Abstract: The study of the mitochondrial DNA (mtDNA) has been hampered by the lack of methods to genetically manipulate the mitochondrial genome in living animal cells. This limitation has been partially alleviated by the ability to transfer mitochondria (and their mtDNAs) from one cell into another, as long as they are from the same species. This is done by isolating mtDNA-containing cytoplasts and fusing these to cells lacking mtDNA. This transmitochondrial cytoplasmic hybrid (cybrid) technology has helped the field understand the mechanism of several pathogenic mutations. In this chapter, we describe procedures to obtain transmitochondrial cybrids.
PubMed ID: 32183971
MeSH Terms: Animals; Cell Line; Cell Line, Tumor; Cell Nucleus/metabolism; Cytological Techniques/methods*; Cytoplasm/metabolism*; Humans; Hybrid Cells/metabolism*; Mice; Mitochondria/metabolism