Skip Navigation

Publication Detail

Title: Quantitative Systems Toxicology Analysis of In Vitro Mechanistic Assays Reveals Importance of Bile Acid Accumulation and Mitochondrial Dysfunction in TAK-875-Induced Liver Injury.

Authors: Longo, Diane M; Woodhead, Jeffrey L; Walker, Paul; Herédi-Szabó, Krisztina; Mogyorósi, Károly; Wolenski, Francis S; Dragan, Yvonne P; Mosedale, Merrie; Siler, Scott Q; Watkins, Paul B; Howell, Brett A

Published In Toxicol Sci, (2019 02 01)

Abstract: TAK-875 (fasiglifam), a GPR40 agonist in development for the treatment of type 2 diabetes (T2D), was voluntarily terminated in Phase III trials due to adverse liver effects. The potential mechanisms of TAK-875 toxicity were explored by combining in vitro experiments with quantitative systems toxicology (QST) using DILIsym, a mathematical representation of drug-induced liver injury. In vitro assays revealed that bile acid transporters were inhibited by both TAK-875 and its metabolite, TAK-875-Glu. Experimental data indicated that human bile salt export pump (BSEP) inhibition by TAK-875 was mixed whereas sodium taurocholate co-transporting polypeptide (NTCP) inhibition by TAK-875 was competitive. Furthermore, experimental data demonstrated that both TAK-875 and TAK-875-Glu inhibit mitochondrial electron transport chain (ETC) enzymes. These mechanistic data were combined with a physiologically based pharmacokinetic (PBPK) model constructed within DILIsym to estimate liver exposure of TAK-875 and TAK-875-Glu. In a simulated population (SimPops) constructed to reflect T2D patients, 16/245 (6.5%) simulated individuals developed alanine aminotransferase (ALT) elevations, an incidence similar to that observed with 200 mg daily dosing in clinical trials. Determining the mode of bile acid transporter inhibition (Ki) was critical to accurate predictions. In addition, simulations conducted on a sensitive subset of individuals (SimCohorts) revealed that when either BSEP or ETC inhibition was inactive, ALT elevations were not predicted to occur, suggesting that the two mechanisms operate synergistically to produce the observed clinical response. These results demonstrate how utilizing QST methods to interpret in vitro experimental results can lead to an improved understanding of the clinically relevant mechanisms underlying drug-induced toxicity.

PubMed ID: 30289550 Exiting the NIEHS site

MeSH Terms: ATP Binding Cassette Transporter, Subfamily B, Member 11/antagonists & inhibitors*; Benzofurans/pharmacokinetics; Benzofurans/toxicity*; Bile Acids and Salts/metabolism*; Chemical and Drug Induced Liver Injury/etiology*; Chemical and Drug Induced Liver Injury/metabolism; Computer Simulation; Humans; Liver/drug effects*; Liver/metabolism; Mitochondria/drug effects*; Mitochondria/metabolism; Models, Biological*; Sulfones/pharmacokinetics; Sulfones/toxicity*

Back
to Top