Title: Autophagy promotes angiogenesis via AMPK/Akt/mTOR signaling during the recovery of heat-denatured endothelial cells.
Authors: Liang, Pengfei; Jiang, Bimei; Li, Yuanbin; Liu, Zhenguo; Zhang, Pihong; Zhang, Minghua; Huang, Xiaoyuan; Xiao, Xianzhong
Published In Cell Death Dis, (2018 11 19)
Abstract: Our previous study demonstrated that angiogenesis increased during the recovery of heat-denatured endothelial cells. However, the mechanism is still unclear. This study aimed to investigate the relation of autophagy and angiogenesis during the recovery of heat-denatured endothelial cells. A rat deep partial-thickness burn model and heat-denatured human umbilical vein endothelial cells (HUVECs) model (52 °C for 35 s) were used. Autophagy increased significantly in the dermis and HUVECs in a time-dependent manner after heat denaturation and recovery for 2-5 days. Rapamycin-mediated autophagy enhanced the pro-angiogenic effect, evidenced by increased proliferation and migration of HUVECs, and formation of tube-like structures. Autophagy inhibition by 3-Methyladenine (3-MA) abolished the angiogenesis in heat-denatured HUVECs after recovery for 3-5 days. Moreover, heat denaturation augmented the phosphorylation of AMP-activated protein kinase (AMPK) but reduced the phosphorylation of Akt and mTOR in HUVECs. Furthermore, autophagy inhibition by antioxidant NAC, compound C or AMPK siRNA impaired cell proliferation, migration and tube formation heat-denatured HUVECs. At last, the in vivo experiments also showed that inhibition of autophagy by bafilomycin A1 could suppress angiogenesis and recovery of heat-denatured dermis.Taken together, we firstly revealed that autophagy promotes angiogenesis via AMPK/Akt/mTOR signaling during the recovery of heat-denatured endothelial cells and may provide a potential therapeutic target for the recovery of heat-denatured dermis.
PubMed ID: 30455420
MeSH Terms: Adenine/analogs & derivatives; Adenine/pharmacology; Animals; Autophagy/genetics*; Cell Proliferation/drug effects*; Hot Temperature; Human Umbilical Vein Endothelial Cells; Humans; Macrolides/pharmacology; Neovascularization, Physiologic/genetics*; Phosphorylation/genetics; Protein Kinases/genetics; Proto-Oncogene Proteins c-akt/genetics; Rats; Signal Transduction/genetics; Sirolimus/pharmacology; TOR Serine-Threonine Kinases/genetics