Title: GLOBAL AND TARGETED PROFILING OF GTP-BINDING PROTEINS IN BIOLOGICAL SAMPLES BY MASS SPECTROMETRY.

Authors: Huang, Ming; Wang, Yinsheng

Published In Mass Spectrom Rev, (2021 05)

Abstract: GTP-binding proteins are among the most important enzyme families that are involved in a plethora of biological processes. However, owing to the enormous diversity of the nucleotide-binding protein family, comprehensive analyses of the expression level, structure, activity, and regulatory mechanisms of GTP-binding proteins remain challenging with the use of conventional approaches. The many advances in mass spectrometry (MS) instrumentation and data acquisition methods, together with a variety of enrichment approaches in sample preparation, render MS a powerful tool for the comprehensive characterizations of the activities and expression levels of various GTP-binding proteins. We review herein the recent developments in the application of MS-based techniques, together with general and widely used affinity enrichment approaches, for the proteome-wide and targeted capture, identification, and quantification of GTP-binding proteins. The working principles, advantages, and limitations of various strategies for profiling the expression level, activity, posttranslational modifications, and interactome of GTP-binding proteins are discussed. It can be envisaged that future applications of MS-based proteomics will lead to a better understanding about the roles of GTP-binding proteins in different biological processes and human diseases. © 2020 John Wiley & Sons Ltd. Mass Spec Rev.

PubMed ID: 32519381

MeSH Terms: Affinity Labels/chemistry; Animals; Biotinylation; Electrophoresis/methods; GTP-Binding Proteins/analysis*; GTP-Binding Proteins/chemistry; GTP-Binding Proteins/isolation & purification; GTP-Binding Proteins/metabolism*; Guanine/chemistry; Humans; Mass Spectrometry/methods*; Protein Processing, Post-Translational; Proteomics/methods*