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Title: Transient Receptor Potential Ankyrin-1 and Vanilloid-3 Differentially Regulate Endoplasmic Reticulum Stress and Cytotoxicity in Human Lung Epithelial Cells After Pneumotoxic Wood Smoke Particle Exposure.

Authors: Nguyen, Nam D; Memon, Tosifa A; Burrell, Katherine L; Almestica-Roberts, Marysol; Rapp, Emmanuel; Sun, Lili; Scott, Abigail F; Rower, Joseph E; Deering-Rice, Cassandra E; Reilly, Christopher A

Published In Mol Pharmacol, (2020 11)

Abstract: This study investigated the roles of transient receptor potential (TRP) ankyrin-1 (TRPA1) and TRP vanilloid-3 (TRPV3) in regulating endoplasmic reticulum stress (ERS) and cytotoxicity in human bronchial epithelial cells (HBECs) treated with pneumotoxic wood smoke particulate matter (WSPM) and chemical agonists of each channel. Functions of TRPA1 and TRPV3 in pulmonary epithelial cells remain largely undefined. This study shows that TRPA1 activity localizes to the plasma membrane and endoplasmic reticulum (ER) of cells, whereas TRPV3 resides primarily in the ER. Additionally, treatment of cells using moderately cytotoxic concentrations of pine WSPM, carvacrol, and other TRPA1 agonists caused ERS as a function of both TRPA1 and TRPV3 activities. Specifically, ERS and cytotoxicity were attenuated by TRPA1 inhibition, whereas inhibiting TRPV3 exacerbated ERS and cytotoxicity. Interestingly, after treatment with pine WSPM, TRPA1 transcription was suppressed, whereas TRPV3 was increased. TRPV3 overexpression in HBECs conferred resistance to ERS and an attenuation of ERS-associated cell cycle arrest caused by WSPM and multiple prototypical ERS-inducing agents. Alternatively, short hairpin RNA-mediated knockdown of TRPV3, like the TRPV3 antagonist, exacerbated ERS. This study reveals previously undocumented roles for TRPA1 in promoting pathologic ERS and cytotoxicity elicited by pneumotoxic WSPM and TRPA1 agonists, and a unique role for TRPV3 in fettering pathologic facets of the integrated ERS response. SIGNIFICANCE STATEMENT: These findings provide new insights into how wood smoke particulate matter and other transient receptor potential ankyrin-1 (TRPA1) and transient receptor potential vanilloid-3 (TRPV3) agonists can affect human bronchial epithelial cells and highlight novel physiological and pathophysiological roles for TRPA1 and TRPV3 in these cells.

PubMed ID: 32938721 Exiting the NIEHS site

MeSH Terms: Cell Line; Cymenes/adverse effects; Endoplasmic Reticulum Stress/drug effects*; Endoplasmic Reticulum/drug effects; Endoplasmic Reticulum/metabolism; Epithelial Cells/drug effects*; Epithelial Cells/metabolism; HEK293 Cells; Humans; Lung/drug effects*; Lung/metabolism; Particulate Matter/administration & dosage*; Pinus/adverse effects; Smoke/adverse effects*; TRPA1 Cation Channel/metabolism*; TRPV Cation Channels/metabolism*; Transient Receptor Potential Channels/metabolism; Wood/adverse effects

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