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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Detection and Quantification of Polychlorinated Biphenyl Sulfates in Human Serum.

Authors: Zhang, Duo; Saktrakulkla, Panithi; Tuttle, Kristopher; Marek, Rachel F; Lehmler, Hans-Joachim; Wang, Kai; Hornbuckle, Keri C; Duffel, Michael W

Published In Environ Sci Technol, (2021 Feb 16)

Abstract: Polychlorinated biphenyls (PCBs) are persistent toxic chemicals with both legacy sources (e.g., Aroclors) and new sources (e.g., unintentional contaminants in some pigments and varnishes). PCB sulfates are derived from further metabolism of hydroxylated PCBs (OH-PCBs), which are oxidative metabolites of PCBs. While OH-PCBs and PCB sulfates are implicated in multiple toxicological effects, studies of PCB sulfates in human serum have been limited by available analytical procedures. We have now developed a method for extraction of PCB sulfates from serum followed by differential analysis with, and without, sulfatase-catalyzed hydrolysis to OH-PCBs. A sulfatase from Helix pomatia was purified by affinity chromatography, and it displayed broad specificity for PCB sulfates without contaminant glucuronidase activity. Following sulfatase-catalyzed hydrolysis of the PCB sulfates extracted from serum, the corresponding OH-PCBs were derivatized to methoxy-PCBs and quantitated by GC-MS/MS. In a pooled sample of human serum, we identified 10 PCB sulfates, with three PCB sulfate congeners exhibiting the highest concentrations from 1200 to 3970 pg/g of serum. In conclusion, we have developed a sensitive and specific method for the determination of PCB sulfates in human serum.

PubMed ID: 33502843 Exiting the NIEHS site

MeSH Terms: Aroclors; Humans; Hydroxylation; Polychlorinated Biphenyls*; Sulfates; Tandem Mass Spectrometry

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