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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Arsenate reductase II. Purine nucleoside phosphorylase in the presence of dihydrolipoic acid is a route for reduction of arsenate to arsenite in mammalian systems.

Authors: Radabaugh, Timothy R; Sampayo-Reyes, Adriana; Zakharyan, Robert A; Aposhian, H Vasken

Published In Chem Res Toxicol, (2002 May)

Abstract: An arsenate reductase has been partially purified from human liver using ion exchange, molecular exclusion, hydroxyapatite chromatography, preparative isoelectric focusing, and electrophoresis. When SDS-beta-mercaptoethanol-PAGE was performed on the most purified fraction, two bands were obtained. One of these bands was a 34 kDa protein. Each band was excised from the gel and sequenced by LC-MS/MS, and sequest analyses were performed against the OWL database SWISS-PROT with PIR. Mass spectra analysis matched the 34 kDa protein of interest with human purine nucleoside phosphorylase (PNP). The peptide fragments equal to 40.1% of the total protein were 100% identical to the corresponding regions of the human purine nucleoside phosphorylase. Reduction of arsenate in the purine nucleoside arsenolysis reaction required both PNP and dihydrolipoic acid (DHLP). The PNP rate of reduction of arsenate with the reducing agents GSH or ascorbic acid was negligible compared to that with the naturally occurring dithiol DHLP and synthetic dithiols such as BAL (British anti-lewisite), DMPS (2,3-dimercapto-1-propanesulfonate), or DTT (alpha-dithiothreitol). The arsenite production reaction of thymidine phosphorylase had approximately 5% of such PNP activity. Phosphorylase b was inactive. Monomethylarsonate (MMAV) was not reduced by PNP. The experimental results indicate PNP is an important route for the reduction of arsenate to arsenite in mammalian systems.

PubMed ID: 12018991 Exiting the NIEHS site

MeSH Terms: Animals; Antioxidants/chemistry*; Arsenite Transporting ATPases; Cattle; Chromatography; Drug Interactions; Electrophoresis; Humans; Ion Pumps/chemistry; Ion Pumps/isolation & purification*; Liver/chemistry*; Male; Mass Spectrometry; Multienzyme Complexes/chemistry; Multienzyme Complexes/isolation & purification*; Purine-Nucleoside Phosphorylase/chemistry; Purine-Nucleoside Phosphorylase/isolation & purification*; Thioctic Acid/analogs & derivatives*; Thioctic Acid/chemistry*

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