Title: Identification and characterization of uptake systems for cystine and cysteine in cultured astrocytes and neurons: evidence for methylmercury-targeted disruption of astrocyte transport.
Authors: Shanker, G; Aschner, M
Published In J Neurosci Res, (2001 Dec 1)
Abstract: Maintenance of appropriate intracellular glutathione (GSH) levels is crucial for cellular defense against oxidative damage. A suggested mechanism of methylmercury (MeHg) neurotoxicity implicates the involvement of oxygen radical formation and a decrease in cellular levels of GSH. Astrocytes play an important role in providing GSH precursors to neurons, and as will be discussed in this review, altered GSH homeostasis likely leads to impairment of astrocytic handling of glutamate, and neuronal energy metabolism. The review summarizes recent observations on transport systems for cysteine and cystine, precursors of GSH, in primary cultures of astrocytes and neurons, and their sensitivity to MeHg treatment.
PubMed ID: 11746429
MeSH Terms: Animals; Astrocytes/drug effects*; Astrocytes/metabolism; Carrier Proteins/drug effects*; Carrier Proteins/metabolism; Central Nervous System/drug effects*; Central Nervous System/metabolism; Central Nervous System/physiopathology; Cysteine/metabolism; Cystine/metabolism; Glutathione/biosynthesis*; Humans; Mercury Poisoning, Nervous System/metabolism*; Mercury Poisoning, Nervous System/physiopathology; Methylmercury Compounds/toxicity*; Neurons/drug effects*; Neurons/metabolism; Research Support, U.S. Gov't, P.H.S.