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Title: Ozonation of chrysene: evaluation of byproduct mixtures and identification of toxic constituent.

Authors: Luster-Teasley, S L; Yao, J J; Herner, H H; Trosko, J E; Masten, S J

Published In Environ Sci Technol, (2002 Mar 01)

Abstract: The effects of chrysene and the ozonated byproducts on in vitro gap junctional intercellular communication (GJIC) were evaluated using the scrape loading/dye transfer (SL/DT) technique. A 1 mM solution of chrysene was ozonated at dosages of 1.75, 3, 4.25, and 5 mol O3/mol chrysene (Chr). The early ozonation mixture, 1.75 mol O3/mol Chr, exhibited greater inhibition to GJIC than chrysene and irreversible damage to cells leading to cell death. To determine the compounds potentially responsible for the increase in toxicity, the byproducts formed upon treatment with 1.44 mol O3/mol Chr were separated into 14 fractions using RP-HPLC. The major compounds identified in the fractions were 2-(2'-formyl) phenyl-1-naphthaldehyde, 2-(2'formyl) phenyl-1-naphthoic acid, and 2-2-carboxyphenyl-1-naphthoic acid. 2-(2'-Formyl) phenyl-1-naphthaldehyde was determined to be the compound causing GJIC inhibition in sample fractions and byproduct mixtures.

PubMed ID: 11918009 Exiting the NIEHS site

MeSH Terms: Animals; Cell Communication/drug effects*; Cell Culture Techniques; Cell Death; Chrysenes/adverse effects*; Chrysenes/chemistry*; Epithelial Cells/drug effects; Epithelial Cells/physiology; Gap Junctions/drug effects*; Oxidants, Photochemical/chemistry*; Oxidation-Reduction; Ozone/chemistry*; Rats

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