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Title: Cell cycle dependence of radiation-induced homologous recombination in cultured monkey cells.

Authors: Cao, J; DePrimo, S E; Stringer, J R

Published In Mutat Res, (1997 Mar 21)

Abstract: A lacZ transgene recombination system that reports homologous recombination events involving duplicated lacZ segments was used to study recombination in monkey cells exposed to ionizing radiation at different points in the cell cycle. With this system, recombination events can be detected in single cells by histochemical staining soon after exposure of cells to DNA-damaging treatment. Ionizing radiation rapidly induced recombination 5-10-fold in cells that were at the mitosis stage of the cell cycle. Irradiation either of cells at other points in the cell cycle or of nonsynchronized cells had less of an effect on recombination between lacZ segments.

PubMed ID: 9100846 Exiting the NIEHS site

MeSH Terms: Animals; Cell Cycle/physiology*; Cell Line; Cell Survival/radiation effects; Dose-Response Relationship, Radiation; Gamma Rays*; Haplorhini; Interphase; Lac Operon/genetics; Mitosis; Recombination, Genetic/radiation effects*; S Phase

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