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Title: Serotonin 5-HT2C receptor stimulates cyclic GMP formation in choroid plexus.

Authors: Kaufman, M J; Hartig, P R; Hoffman, B J

Published In J Neurochem, (1995 Jan)

Abstract: The serotonin 5-HT2C receptor (formerly designated the 5-HT1C receptor) of the choroid plexus triggers phosphoinositide turnover. In the present study, we demonstrate that receptor activation also triggers the formation of cyclic GMP (cGMP). Application of 1 microM 5-HT to porcine choroid plexus tissue slices resulted in stimulation of cGMP formation to a maximum of five-fold basal level, with an EC50 of 11 nM. This response was not inhibited by muscarinic or beta-adrenergic receptor antagonists. Serotonin receptor antagonists inhibited cGMP formation with apparent Ki values of 1.3 (mianserin), 200 (ketanserin), and 5,500 (spiperone) nM, respectively. Neither serotonin-stimulated cGMP formation nor PI turnover was inhibited by pertussis toxin pretreatment. Preliminary biochemical studies suggested that serotonin-stimulated cGMP formation was calcium, phospholipase A2, and lipoxygenase dependent, as incubation in low calcium buffers or inclusion of the phospholipase A2 or lipoxygenase inhibitors p-bromophenacylbromide or BW 755c resulted in significant reduction of cGMP formation. The present results suggest that in addition to triggering phosphoinositide turnover, choroid plexus serotonin 5-HT2C receptors trigger cGMP formation in a calcium-sensitive manner.

PubMed ID: 7798914 Exiting the NIEHS site

MeSH Terms: Animals; Cattle; Choroid Plexus/drug effects; Choroid Plexus/metabolism*; Cyclic GMP/metabolism*; Ketanserin/pharmacology; Mianserin/pharmacology; Phosphatidylinositols/metabolism; Receptors, Serotonin/physiology*; Spiperone/pharmacology; Swine

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