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National Institute of Environmental Health Sciences

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Publication Detail

Title: Hybrid nanoparticles based on organized protein immobilization on fullerenes.

Authors: Nednoor, Pramod; Capaccio, Marcello; Gavalas, Vasilis G; Meier, Mark S; Anthony, John E; Bachas, Leonidas G

Published In Bioconjug Chem, (2004 Jan-Feb)

Abstract: Nanoscale carbon materials (i.e., fullerenes and nanotubes) are an attractive platform for applications in biotransformations and biosensors. The interesting properties displayed by nanoparticles demand new strategies for the manipulation of these materials on the nanoscale. Controlled modification of their surface with biomolecules is required to fully realize their potential in bionanotechnology. In this work, immobilization of a fullerene derivative with a mutant subtilisin is demonstrated, and the effect of the fullerene on the protein activity is determined. The fullerene-conjugated enzyme had improved catalytic properties in comparison to subtilisin immobilized on nonporous silica. Further, the pH profile of free and fullerene-conjugated subtilisin were almost identical.

PubMed ID: 14733578 Exiting the NIEHS site

MeSH Terms: Enzymes, Immobilized; Fullerenes/chemistry*; Hydrogen-Ion Concentration; Microspheres*; Nanotechnology; Protein Engineering; Proteins/chemistry*; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.; Subtilisin/chemistry*; Surface Properties

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