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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Selection and partial characterization of a Pseudomonas aeruginosa mono-rhamnolipid deficient mutant.

Authors: Wild, M; Caro, A D; Hernández, A L; Miller, R M; Soberón-Chávez, G

Published In FEMS Microbiol Lett, (1997 Aug 15)

Abstract: Pseudomonas aeruginosa produces rhamnolipids which are tenso-active compounds with potential industrial and environmental applications. There are two main types of rhamnolipids produced in liquid cultures, rhamnosyl-beta-hydroxydecanoyl-beta-hydroxydecanoate (mono-rhamnolipid) and rhamnosyl-rhamnosyl-beta-hydroxydecanoyl-beta-hydroxyd ecanoate (di-rhamnolipid). In this work we report the selective isolation of a rhamnolipid deficient mutant (IBT8), which does not accumulate mono-rhamnolipid while still producing di-rhamnolipid. IBT8 was selected after random mutagenesis with Tn501; yet, its mono-rhamnolipid deficiency was found associated neither with its Tn501 insertion nor with a possible alteration in the rhlABRI genes for rhamnosyl-transferase 1 synthesis. Different possibilities to explain IBT8 phenotype are discussed.

PubMed ID: 9271853 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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