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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Using fluorescently labelled M13-tailed primers to isolate 45 novel microsatellite loci from the arboviral vector Culex tarsalis.

Authors: Venkatesan, M; Hauer, M C; Rasgon, J L

Published In Med Vet Entomol, (2007 Jun)

Abstract: Culex tarsalis Coquillett (Diptera: Culicidae) is a highly efficient arbovirus vector. Spatial and temporal heterogeneity have been observed in Cx tarsalis for phenotypic traits including autogeny, virus susceptibility and host preference. Genetic differences between populations may in part explain these observations. Using the M13-tailed primer method, we identified 45 novel polymorphic microsatellite markers from microsatellite-enriched Cx tarsalis genomic libraries. The M13-tailed primer method was more efficient in identifying useful loci than traditional screening by acrylamide gel electrophoresis. These markers will be useful for investigating genetic questions in this important vector mosquito.

PubMed ID: 17550440 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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