Skip Navigation

Publication Detail

Title: Identification of the peroxidation products of 13-hydroxy-gamma-linolenate and 15-hydroxyarachidonate: mechanistic studies on the formation of leukotriene-like diols.

Authors: Rector, Christopher L; Stec, Donald F; Brash, Alan R; Porter, Ned A

Published In Chem Res Toxicol, (2007 Nov)

Abstract: Monohydroxy-gamma-linolenates and arachidonates were oxidized in the presence of alpha-tocopherol and free radical initiators at 37 degrees C. The dihydroxylinolenate products were analyzed and identified by use of a combination of liquid chromatography, mass spectrometry, and NMR techniques. A mechanism for the formation of the dihydroxylinolenates is proposed based on product analysis of oxidations using varied concentrations of alpha-tocopherol. The mechanism for monohydroxyarachidonate oxidation is the same as that of monohydroxylinolenates. However, arachidonate diol analysis is more complicated because of the formation of additional regioisomers that are a result of the parent arachidonate possessing multiple bisallylic hydrogens.

PubMed ID: 17907784 Exiting the NIEHS site

MeSH Terms: Arachidonic Acids/metabolism*; Chromatography, High Pressure Liquid; Leukotrienes/metabolism; Linoleic Acids/metabolism*; Lipid Peroxidation*; Magnetic Resonance Spectroscopy; Mass Spectrometry; Oxidation-Reduction

Back
to Top