Title: Absence of 60-Hz, 0.1-mT magnetic field-induced changes in oncogene transcription rates or levels in CEM-CM3 cells.

Authors: Jahreis, G P; Johnson, P G; Zhao, Y L; Hui, S W

Published In Biochim Biophys Acta, (1998 Dec 22)

Abstract: Our objective was to assess the reproducibility of the 60-Hz magnetic field-induced, time-dependent transcription changes of c-fos, c-jun and c-myc oncogenes in CEM-CM3 cells reported by Phillips et al. (Biochim. Biophys. Acta, 1132 (1992) 140-144). Cells were exposed to a 60-Hz magnetic field (MF) at 0.1 mT (rms), generated by a pair of Helmholtz coils energized in a reinforcing (MF) mode, or to a null magnetic field when the coils were energized in a bucking (sham) mode. After MF or sham exposure for 15, 30, 60 or 120 min, nuclei and cytoplasmic RNA were extracted. Transcription rates were measured by a nuclear run-on assay, and values were normalized against either their zero-time exposure values, or against those of the c-G3PDH (housekeeping) gene at the same time points. There was no significant difference, at P=0.05, detected between MF and either sham-exposed or control cells at any time point. Transcript levels of the oncogenes were measured by Northern analysis and normalized as above. No significant difference (P=0.05) in transcript levels between MF and either sham-exposed or control cells was detected.

PubMed ID: 9878814

MeSH Terms: Electromagnetic Fields*; Gene Expression Regulation, Neoplastic; Humans; Oncogenes/genetics*; Proto-Oncogene Proteins c-fos/genetics; Proto-Oncogene Proteins c-jun/genetics; Proto-Oncogene Proteins c-myc/genetics; RNA/genetics; RNA/metabolism*; Transcription, Genetic; Tumor Cells, Cultured/cytology; Tumor Cells, Cultured/metabolism