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National Institute of Environmental Health Sciences

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Publication Detail

Title: The NH2-terminal region of rabbit CYP2E1 is not essential for interaction with NADPH-cytochrome P450 reductase.

Authors: Voznesensky, A I; Schenkman, J B; Pernecky, S J; Coon, M J

Published In Biochem Biophys Res Commun, (1994 Aug 30)

Abstract: Recently we reported that electrostatic forces interfere with the formation of the NADPH-cytochrome P450 reductase/cytochrome P450 electron transfer complex and suggested that this complex is formed by the attraction of the complementary hydrophobic patches (Voznesensky, A., and Schenkman, J. (1994) 269 J. Biol. Chem, 15724-1573). In this report we evaluate the role of the NH2-terminal hydrophobic region of CYP2E1 in the interaction with the reductase by comparing ionic strength dependence of the reduction of the full-length and truncated delta 3-29 CYP2E1. Increasing ionic strength stimulates reduction of both full-length and truncated CYP2E1. The neutralization of electrostatic interactions by increasing ionic strength revealed no impairment of the delta 3-29 CYP2E1 reduction compared to that of the full-length CYP2E1, indicating that the NH2-terminal region is not essential for the interaction of the cytochrome with the reductase.

PubMed ID: 8074649 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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