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National Institute of Environmental Health Sciences

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Publication Detail

Title: Acetanilide 4-hydroxylase and acetanilide 2-hydroxylase activity in hepatic microsomes from induced mice.

Authors: Lewandowski, M; Chui, Y C; Levi, P; Hodgson, E

Published In Toxicol Lett, (1991 Feb)

Abstract: A simple and sensitive method for the separation of 14C-labelled acetanilide, 4-hydroxyacetanilide, 3-hydroxyacetanilide and 2-hydroxyacetanilide was developed using thin-layer chromatography. This separation is the basis for the assay of acetanilide 4-hydroxylase and acetanilide 2-hydroxylase activity in liver microsomes from DBA2/N male mice that had been treated with phenobarbital, 3-methylcholanthrene, isosafrole or n-butylbenzodioxole. Microsomes were incubated with [14C]acetanilide and extracted with benzene and ethyl acetate. The extract was applied to silica gel plates and developed with a hexane/isopropanol/ammonium hydroxide/water solvent system. The radiolabelled phenolic metabolites and the parent compound were detected using a Berthold Automatic TLC Linear Analyzer. Although the 4-hydroxylated metabolite was the primary product detected, this method can be used to detect other phenolic metabolites.

PubMed ID: 1998210 Exiting the NIEHS site

MeSH Terms: Acetanilides/isolation & purification; Acetanilides/metabolism*; Animals; Aryl Hydrocarbon Hydroxylases/metabolism*; Chromatography, Thin Layer; Male; Mice; Mice, Inbred DBA; Microsomes, Liver/drug effects; Microsomes, Liver/enzymology; Microsomes, Liver/metabolism*; Safrole/pharmacology

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