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Publication Detail

Title: Isolation and characterization of the major glycosphingolipids from the liver of the rainbow trout (Oncorhynchus mykiss): identification of an abundant source of 9-O-acetyl GD3.

Authors: Ostrander, G K; Bozlee, M; Fukuda, M; Dell, A; Thomas-Oates, J E; Levery, S B; Eaton, H L; Hakomori, S; Holmes, E H

Published In Arch Biochem Biophys, (1991 Feb 01)

Abstract: The carbohydrate structures of the major glycosphingolipids from the liver of the rainbow trout Oncorhynchus mykiss have been examined. We have isolated and identified four major neutral (glucosylceramide, galactosylceramide, lactosylceramide, and globoside) and five acidic (sulfatide, GM3, GM2, GD1a, and 9-O-Acetyl GD3) glycosphingolipids from trout liver. They have been characterized by 1H nuclear magnetic resonance spectroscopy, methylation analysis, fast atom bombardment mass spectrometry, and specific monoclonal antibodies. Significantly, the relatively scarce ganglioside 9-O-acetyl GD3 was found to comprise approximately 23% of the total ganglioside content of normal rainbow trout liver. 9-O-Acetyl GD3 is, however, abundant in human melanoma and as such, trout liver may be a suitable source of this antigen.

PubMed ID: 1989525 Exiting the NIEHS site

MeSH Terms: Acetylation; Animals; Carbohydrate Sequence; Gangliosides/analysis*; Glycosphingolipids/analysis*; Hydrolysis; Liver/chemistry*; Methylation; Molecular Sequence Data; Trout*

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