Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Nitrate biosynthesis in rats, ferrets and humans. Precursor studies with L-arginine.

Authors: Leaf, C D; Wishnok, J S; Hurley, J P; Rosenblad, W D; Fox, J G; Tannenbaum, S R

Published In Carcinogenesis, (1990 May)

Abstract: L-Arginine, the primary nitrogen source for nitric oxide synthesized by many cell types in culture and for biosynthesized nitrate in humans, is also a nitrogen source for biosynthesized nitrate in rats and ferrets. After administration of [15N2]L-arginine to rats and ferrets, [15N]NO3- was detected in urine. Escherichia coli lipopolysaccharide induced more than a 10-fold increase in urinary nitrate in rats and a parallel increase in incorporation of 15N from [15N2]L-arginine into NO3-. Bradykinin, a vasodilator which induces nitric oxide production by endothelial cells in vitro, lacked detectable effect on urinary nitrate or on incorporation of L-arginine nitrogen into nitrate in rats. A prolonged period of vasodilation brought on by an extended period of exercise increased urinary nitrate 2-fold in human subjects. In the rat, recoveries in 24 h post-dose urine collections of [15N]NO3- given i.v. and i.p. were 75 and 64% respectively, while in the ferret, recoveries of i.v. and per os [15N]NO3- doses were 49 and 34% respectively. Thus, nitrate synthesized by mammalian cells in vivo would undergo losses similar to those for exogenous nitrate.

PubMed ID: 2335012 Exiting the NIEHS site

MeSH Terms: Adult; Animals; Arginine/metabolism; Exercise/physiology; Ferrets; Humans; Injections, Intraperitoneal; Injections, Intravenous; Male; Nitrates/administration & dosage; Nitrates/metabolism*; Nitrogen Radioisotopes; Nitrogen/metabolism; Rats; Rats, Inbred Strains

to Top