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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Cultured myometrial cells establish communicating gap junctions.

Authors: Caruso, R L; Juberg, D R; Caldwell, V; Corcos, I A

Published In Cell Biol Int Rep, (1990 Oct)

Abstract: Myometrial cells were isolated and cultured from term rat uterus. The myometrial origin of the cultures was verified by antibody staining of cellular desmin and alpha-smooth muscle actin. The presence of functional gap junctions was indicated by transfer of radiolabeled nucleotide and microinjected Lucifer yellow dye. The cultured cells expressed mRNA recognized by a connexin43 gap junction cDNA probe. To our knowledge, this is the first report that isolated myometrial cells form gap junctions in culture.

PubMed ID: 2176132 Exiting the NIEHS site

MeSH Terms: Actins/analysis; Animals; Biomarkers; Cells, Cultured; Connexins; DNA Probes; Desmin/analysis; Female; Intercellular Junctions/ultrastructure*; Isoquinolines/metabolism; Membrane Proteins/analysis; Myometrium/cytology*; Pregnancy; RNA, Messenger/analysis; Rats; Rats, Inbred Strains

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