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Title: The flavin-containing monooxygenase enzymes expressed in rabbit liver and lung are products of related but distinctly different genes.

Authors: Lawton, M P; Gasser, R; Tynes, R E; Hodgson, E; Philpot, R M

Published In J Biol Chem, (1990 Apr 05)

Abstract: Preparations of mRNA isolated from rabbit lung and liver were used in the construction of libraries that were screened for cDNAs encoding the pulmonary or hepatic isozyme of the flavin-containing monooxygenase. The hepatic library was screened with cDNA encoding the flavin-containing monooxygenase expressed in pig liver, and a clone containing a 2.0-kilobase insert was detected and isolated. This cDNA insert encoded a protein of 535 amino acids with a primary structure 87% identical to that of the pig flavin-containing monooxygenase. The pulmonary library was screened with polyclonal antibodies to the flavin-containing monooxygenase expressed in rabbit lung, and a clone containing a 2.6-kilobase insert was detected and isolated. Although the protein encoded by this insert also contained 535 amino acids, its primary sequence was only 56% identical to that of the liver enzyme. The sequences of several peptides obtained by digestion of the purified rabbit pulmonary flavin-containing monooxygenase with trypsin matched exactly with sequences derived from the cDNA structure. Tissue-specific distribution of mRNA for the hepatic and pulmonary isozymes of the flavin-containing monooxygenase was consistent with the distribution of protein, an indication that expression of flavin-containing monooxygenase is controlled at the level of transcription. Analysis of genomic DNA indicates that both the hepatic and pulmonary enzymes may be products of single genes.

PubMed ID: 2318837 Exiting the NIEHS site

MeSH Terms: Amino Acid Sequence; Animals; Base Sequence; Binding Sites; DNA/genetics; DNA/isolation & purification; Flavin-Adenine Dinucleotide/metabolism; Gene Expression*; Isoenzymes/genetics*; Kidney/analysis; Liver/analysis*; Lung/analysis*; Molecular Sequence Data; NADP/metabolism; Nucleic Acid Hybridization; Oxygenases/genetics*; RNA, Messenger/genetics; RNA, Messenger/isolation & purification; Rabbits; Sequence Homology, Nucleic Acid; Swine; Tissue Distribution

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