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Title: Determination of multiple forms of cytochrome P-450 in microsomes from the digestive gland of Cryptochiton stelleri.

Authors: Schlenk, D; Buhler, D R

Published In Biochem Biophys Res Commun, (1989 Aug 30)

Abstract: Polyclonal antibodies raised against purified trout cytochromes P-450 (P-450) LM2 (anti-LM2) and LM4b (anti-LM4b) were used in Western blot analyses with digestive gland microsomes from control and beta-naphthoflavone (BNF)-treated gumboot chitons Cryptochiton stelleri. An increase and decrease in staining intensity subsequent to treatment with anti-LM4b and anti-LM2, respectively, was observed in digestive gland microsomes from BNF-treated chiton. Thus, there appears to be at least two forms of P-450 in microsomes from the digestive gland of Cryptochiton; one of which is induced by BNF and perhaps is involved in benzo(a)pyrene (BP) biotransformation, and another form which is inhibited by BNF.

PubMed ID: 2775279 Exiting the NIEHS site

MeSH Terms: Animals; Benzoflavones; Blotting, Western; Cytochrome P-450 Enzyme System/analysis*; Enzyme Induction/drug effects; Microsomes/enzymology*; Mollusca/enzymology*; beta-Naphthoflavone

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