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Principal Investigator: Papautsky, Ian
Institute Receiving Award University Of Illinois At Chicago
Location Chicago, IL
Grant Number R33ES024717
Funding Organization National Institute of Environmental Health Sciences
Award Funding Period 01 Mar 2015 to 31 Jul 2023
DESCRIPTION (provided by applicant): : Metals are ubiquitous in the environment and have long been recognized to pose significant threats to hu- man health. Blood lead (Pb) has been consistently associated with deficits in IQ and academic achievement in numerous controlled studies. Manganese (Mn) is an essential element, yet neurotoxic in excess, capable of crossing the blood-brain barrier and accumulating in the brain. Current approaches for measuring such exposures suffer from high costs including extensive labor, equipment and time-consuming laboratory procedures, and often, demonstrate long turnaround times. Hence, there is a critical need for improved technology to quantitatively assess levels and identify adverse consequences of these exposures. Our preliminary data show feasibility of measuring of Mn and Pb metals with point-of-care sensors. We therefore propose to demon- strate rapid, point-of-care, multi-analyte assessment of Mn and Pb metals in a finger prick of blood from children. In the R21 phase, we will demonstrate sensor validity and usability for metal analysis in whole blood. The feasibility of the sensor has been established in our prior work. In Aim 1, we will firs demonstrate ability to ac- accurately and reliably measure Mn and Pb metals in blood under real-world conditions with appropriate specific- ty and sensitivity. Then, we will demonstrate ability to scale-up manufacturing of sensors for the study in the R33 phase. In Aim 2, we will develop a streamlined, user-friendly software interface. Finally, we will assess usability of the sensor and supporting documentation by seeking feedback from experts and study staff. We will demonstrate the ability for sensors to be used with minimal oversight and establish strategies for data analysis and deployment. The R33 phase will further demonstrate the added scientific value. In Aim 3 we will deploy the sensor in longitudinal cohort of adolescent children to evaluate the relationship between traditional and novel internal dose markers of metals (Mn and Pb) and neuromotor performance. We will evaluate the novel hypothesis that Mn and Pb will significantly impact functional gait and static and dynamic balance in an adolescent cohort. Additionally, we propose a high risk/high benefit aim to evaluate the clinical significance of unbound or free Mn in whole blood as a novel biomarker for Mn. In Aim 4 we will develop effective participant report-back mecha- nisms to return blood metal results. The R33 phase will culminate with the development of ethical strategies for effective mechanisms for reporting measurement results back to study participants. A relatively swift, simultaneous assessment of heavy metal exposure, which ultimately reduces costs and turnaround times, would benefit pediatric public health nationally and internationally. Indeed, our long-term goal is to use sensors to move blood analysis from the laboratory to the clinical setting, providing results within minutes.
Science Code(s)/Area of Science(s) Primary: 74 - Biosensors/Biomarkers
Secondary: -
Publications See publications associated with this Grant.
Program Officer Yuxia Cui