Title: Comparative properties of purified cytochrome P-448 from beta-naphthoflavone treated rats and rainbow trout.

Authors: Williams, D E; Buhler, D R

Published In Comp Biochem Physiol C Comp Pharmacol Toxicol, (1983)

Abstract: 1. Cytochrome P-448 from beta-naphthoflavone treated rainbow trout (Salmo gairdnerii) liver was purified and compared to purified P-448 from beta-naphthoflavone treated rats (Rattus rattus) and purified P-450 from phenobarbital induced rats. 2. The two P-448 forms had similar spectral properties, substrate specificity, sensitivity to inhibitors and regioselectivity in the metabolism of benzo(a)pyrene and testosterone. 3. Rat and trout P-448 differed in apparent monomeric mol. wt (Mr) by at least 2000 daltons, and did not share identical antigenic determinants. Both rat and trout P-448 were shown to be quite different from rat P-450 using all of the above criteria for distinguishing multiple forms.

PubMed ID: 6191918

MeSH Terms: Animals; Benzo(a)pyrene; Benzoflavones/pharmacology*; Benzopyrene Hydroxylase/metabolism; Benzopyrenes/metabolism; Cytochrome P-450 CYP1A2; Cytochrome P-450 Enzyme System/metabolism; Cytochromes/antagonists & inhibitors; Cytochromes/immunology; Cytochromes/metabolism*; Epitopes/immunology; Flavonoids/pharmacology*; Microsomes, Liver/analysis; Phenobarbital/pharmacology; Rats; Salmonidae/metabolism*; Species Specificity; Spectrophotometry; Substrate Specificity; Testosterone/metabolism; Trout/metabolism*; beta-Naphthoflavone