Title: Single-nucleotide resolution dynamic repair maps of UV damage in Saccharomyces cerevisiae genome.
Authors: Li, Wentao; Adebali, Ogun; Yang, Yanyan; Selby, Christopher P; Sancar, Aziz
Published In Proc Natl Acad Sci U S A, (2018 04 10)
Abstract: We have adapted the eXcision Repair-sequencing (XR-seq) method to generate single-nucleotide resolution dynamic repair maps of UV-induced cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts in the Saccharomyces cerevisiae genome. We find that these photoproducts are removed from the genome primarily by incisions 13-18 nucleotides 5' and 6-7 nucleotides 3' to the UV damage that generate 21- to 27-nt-long excision products. Analyses of the excision repair kinetics both in single genes and at the genome-wide level reveal strong transcription-coupled repair of the transcribed strand at early time points followed by predominantly nontranscribed strand repair at later stages. We have also characterized the excision repair level as a function of the transcription level. The availability of high-resolution and dynamic repair maps should aid in future repair and mutagenesis studies in this model organism.
PubMed ID: 29581276
MeSH Terms: DNA Damage/radiation effects*; DNA Repair; DNA, Fungal/genetics; DNA, Fungal/metabolism; Genome, Fungal/radiation effects*; Pyrimidine Dimers/genetics; Pyrimidine Dimers/metabolism; Saccharomyces cerevisiae/genetics*; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae/radiation effects*; Transcription, Genetic; Ultraviolet Rays