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Publication Detail

Title: Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery.

Authors: Tonzi, Peter; Yin, Yandong; Lee, Chelsea Wei Ting; Rothenberg, Eli; Huang, Tony T

Published In Elife, (2018 11 13)

Abstract: DNA replication stress is often defined by the slowing or stalling of replication fork progression leading to local or global DNA synthesis inhibition. Failure to resolve replication stress in a timely manner contribute toward cell cycle defects, genome instability and human disease; however, the mechanism for fork recovery remains poorly defined. Here, we show that the translesion DNA polymerase (Pol) kappa, a DinB orthologue, has a unique role in both protecting and restarting stalled replication forks under conditions of nucleotide deprivation. Importantly, Pol kappa-mediated DNA synthesis during hydroxyurea (HU)-dependent fork restart is regulated by both the Fanconi Anemia (FA) pathway and PCNA polyubiquitination. Loss of Pol kappa prevents timely rescue of stalled replication forks, leading to replication-associated genomic instability, and a p53-dependent cell cycle defect. Taken together, our results identify a previously unanticipated role for Pol kappa in promoting DNA synthesis and replication stress recovery at sites of stalled forks.

PubMed ID: 30422114 Exiting the NIEHS site

MeSH Terms: Cell Line; DNA Replication*; DNA-Directed DNA Polymerase/metabolism*; DNA/biosynthesis*; Fanconi Anemia Complementation Group Proteins/metabolism; Humans; Hydroxyurea/metabolism; Proliferating Cell Nuclear Antigen/metabolism

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