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National Institute of Environmental Health Sciences

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Publication Detail

Title: Pharmacological challenges examining the underlying mechanism of altered response inhibition and attention due to circadian disruption in adult Long-Evans rats.

Authors: Balachandran, Rekha C; Hatcher, Katherine M; Sieg, Megan L; Sullivan, Emma K; Molina, Leonardo M; Mahoney, Megan M; Eubig, Paul A

Published In Pharmacol Biochem Behav, (2020 06)

Abstract: Endogenous circadian rhythms govern behavior and physiology, while circadian disruption is an environmental factor that impacts cognition by altering the circadian clock at a molecular level. We modeled the effects of 2 sources of circadian disruption - activity occurring during typical rest periods and untimely light exposure - to evaluate the effects of circadian disruption on behavior and underlying neurochemistry. Firstly, adult Long-Evans rats of both sexes were maintained on a 12 h:12 h light:dark cycle and tested using a 5-choice serial reaction time task (5-CSRTT) under 3 conditions: 4 h into the dark phase with no exposure to ambient light during testing (control), 4 h into the dark phase with exposure to ambient light during testing, and 4 h into the light phase. Both models resulted in impulsive behavior and reduced attention compared to control. We established that changes in the diurnal expression pattern occur in the clock gene Period 2 (Per2) in the light phase-tested model. Choline acetyltransferase (Chat) and Dopamine receptor 1 (Drd1) showed rhythmic expression with peak expression during the dark phase regardless of light-testing condition. Next, we performed drug challenges in a new rat cohort to examine the interaction between the cholinergic and dopaminergic neurotransmitter systems in regulating the behavioral changes caused by circadian disruption. We administered the cholinergic agonist nicotine and either the dopamine-1 receptor (DR1) antagonist SCH23390 or the DR2 antagonist eticlopride under the 3 circadian conditions to identify differential drug responses between treatment groups. Rats in both models demonstrated increased sensitivity to nicotine as compared to control, while SCH23390 and eticlopride ameliorated the effect of nicotine on 5-CSRTT performance in both models. Our study is the first to identify detrimental effects of both models of circadian disruption on impulsive behavior, and that the effects of circadian disruption are mediated by an interaction between cholinergic and dopaminergic systems.

PubMed ID: 32224058 Exiting the NIEHS site

MeSH Terms: Animals; Attention*; Benzazepines/pharmacology; Choline O-Acetyltransferase/genetics; Chronobiology Disorders/genetics*; Chronobiology Disorders/physiopathology; Circadian Clocks/genetics*; Circadian Rhythm/genetics*; Cognition; Disease Models, Animal; Dopamine Antagonists/pharmacology; Female; Gene Expression; Impulsive Behavior/drug effects*; Male; Nicotine/pharmacology*; Nicotinic Agonists/pharmacology*; Period Circadian Proteins/genetics; Rats; Rats, Long-Evans; Reaction Time/drug effects; Receptors, Dopamine D1/antagonists & inhibitors; Receptors, Dopamine D1/genetics; Salicylamides/pharmacology

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