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Your Environment. Your Health.

Progress Reports: Boston University: Developmental Toxicity of non-Dioxin-like PCBs and Chemical Mixtures

Superfund Research Program

Developmental Toxicity of non-Dioxin-like PCBs and Chemical Mixtures

Project Leader: John J. Stegeman (Woods Hole Oceanographic Institution)
Co-Investigator: Jared V. Goldstone (Woods Hole Oceanographic Institution)
Grant Number: P42ES007381
Funding Period: 2000-2017
View this project in the NIH Research Portfolio Online Reporting Tools (RePORT)

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Progress Reports

Year:   2016  2015  2014  2013  2012  2010  2009  2008  2007  2006  2005  2004  2003  2002  2001  2000 

Project investigators focused on the cloning, expression and functional characterization of several target cytochrome P450 genes. Novel CYP1 family genes initially assigned to CYP1B were confirmed as a new subfamily, CYP1C, in an alternate teleost fish model (scup). CYP1C genes were also identified in zebrafish and Fundulus. Novel CYP2 family genes in a new subfamily, CYP2AA, were also identified in zebrafish. CYP2AA1 and CYP2AA2 were cloned and expressed in COS cells and functional characterization begun. The CYP2AAs are hoped to be the elusive teleost CYP2B homologues. Chemical effects on expression have been studied with zebrafish cDNA microarrays developed in the lab. These results confirmed that CYP1A is the single most responsive gene in embryos exposed to TCDD. TCDD caused strong changes in expression of 12 known genes and at least 21 other genes. In the brain of developing zebrafish, expression in the vasculature was linked to changes in blood flow and apoptosis.

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