Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Internet Explorer is no longer a supported browser.

This website may not display properly with Internet Explorer. For the best experience, please use a more recent browser such as the latest versions of Google Chrome, Microsoft Edge, and/or Mozilla Firefox. Thank you.

Your Environment. Your Health.

Progress Reports: Duke University: Zebrafish as a Detector and Discriminator of Organophosphate Exposure

Superfund Research Program

Zebrafish as a Detector and Discriminator of Organophosphate Exposure

Project Leaders: Elwood A. Linney (Duke University Medical Center), Richard T. Di Giulio
Grant Number: P42ES010356
Funding Period: 2000-2011

Learn More About the Grantee

Visit the grantee's eNewsletter page Visit the grantee's eNewsletter page Visit the grantee's Twitter page Visit the grantee's Instagram page Visit the grantee's Facebook page Visit the grantee's Video page

Progress Reports

Year:   2008  2007  2006  2005  2004  2003  2002  2001  2000 

Considerable progress has been made this year.  Dr. Linney’s research team has clear evidence that acetylcholine esterase is a target of chlorpyrifos in the developing zebrafish.  Independently inhibiting acetylcholine esterase with morpholinos also produces adults with learning deficiencies and the researchers will see if they can confirm this connection by using heterozygous mutants of acetylcholine esterase.  The supplement awarded to the researchers that allowed for microarray analysis revealed that 15-17 genes that were up-regulated by 3 day 100ng/ml chlorpyrifos exposure were also up-regulated at similar ratios in 10ng/ml and aceytylcholine esterase morpholino injected embryos suggesting an overlap at the gene expression level.

Technologically the research team also added the Noldus Ethovison video tracking/analysis software that allows them to detect motility changes produced from chlorpyrifos exposure.  This should provide a more efficient means of identifying the critical window of vulnerability so that they can efficiently examine events occurring within this window to try to get closer to mechanism.

to Top