Superfund Research Program
PFAS Compound Effects on Metabolic Abnormalities in Rodents
The PFASs Compound Effects on Metabolic Abnormalities in Rodents Project is setting up or optimizing some of the main tools and techniques needed for the next five years (i.e., cell culture, LC-MS, lipid staining, gene expression, and hormone measurement). The researchers will test a wide range of legacy and emerging poly- and perfluorinated alkyl substances (PFASs) (PFBS, PFHxS, PFOS, PFBA, PFHxA, PFHpA, PFOA, PFNA, PFDA, C11 or C12, and GenX) for their effect and potency in human pre-adipocytes. A method to detect the same suite of PFASs in mice tissues will be optimized as to start being ready for conducting a larger developmental study on effects of PFASs in their rodent facility. The PFASs will be tested for ability to alter expression of genes related to lipid metabolism, toxicity to the hepatocytes, and also ability to increase fat content. The researchers' LC-MS method is nearly optimized for extraction and quantification of various PFASs from liver tissue and hepatocytes in collaboration with 3M scientists. First measurements of physico-chemical partitioning will be performed, with an initial focus on critical micelle concentrations and protein, lipid, and octanol-water partitioning constants. The combination of physico-chemical partitioning and bioassays will help the Project understand the mechanisms and effects of PFASs in cells, organs, and animals.