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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Patent Detail: Bioassay for Detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like Compounds and Novel Recombinant Cell Line Useful Therefore

Patent Detail

Superfund Research Program

Bioassay for Detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like Compounds and Novel Recombinant Cell Line Useful Therefore

Number: 5,854,010
Year: 1998
Patent Status: Issued
Authors: Denison, M.S., A. Brouwer, G.C. Clark

A recombinant cell line deposited at the American Type Culture Collection under ATCC accession number CRL-12286, also known as the mouse H1L1.1 cell line, has been made by using genetic engineering techniques for inserting dioxin responsive elements upstream of a luciferase reporter gene, and then, transfecting the resultant recombinant expression plasmid, identified as pGudLuc1.1, into mouse hepatoma cells. The mouse H1L1.1 cell line is useful in a method for the quantitative analysis of polyaromatic hydrocarbons in samples.

Associated Projects:

  • University of California-Davis: Development and Application of Integrated In Vitro and Cell-Based Bioassays
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