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Your Environment. Your Health.

University of Florida

Superfund Research Program

Bioavailability of Chlorinated Hydrocarbons

Project Leader: Margaret O. James
Grant Number: P42ES007375
Funding Period: 1995-2006

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Project Summary (1995-2000)

The objectives of this project are to obtain a better understanding of the factors that influence intestinal bioavailability and biotransformation of lipophilic xenobiotics that may be present in the diet, focussing on halogenated Superfund chemicals. Researchers propose to test the hypotheses that Ah receptor agonists found in Superfund sites cause alterations in the physiological and structural make-up of intestinal cells that affect the intestinal bioavailability and biotransformation of lipophilic chemicals. The first specific aim is to examine the intestinal biotransformation and bioavailability of radiolabeled 3,4,3',4'-tetrachlorobiphenyl (TCB) and 2,4,5,2',4',5'-hexachlorobiphenyl (HCB) as well as the chlorinated solvent trichloroethylene in control and induced catfish. The second specific aim relates to mechanisms of absorption. Scientists propose to investigate the expression of selected proteins, P-glycoprotein and GSH-S-transferase (GST) that may be affected by Ah receptor agonists and involved in the transport of lipophilic xenobiotics. These experiments will use molecular biological techniques with control and TCDD- or TCB-treated fish.
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