Superfund Research Program
Bioanalytical and Statistics Core
Project Leader: Daniel J. Tancredi
Grant Number: P42ES004699
Funding Period: 2017-2022
The Bioanalytical and Statistics Core provides continuous support to the other University of California, Davis (UC Davis) SRP Center projects and cores for the proper design of experiments as well as crucial statistical analysis of data (McReynolds et al, 2019; Pessah et al, 2019). The Core is also providing bioanalytical tools (cell-based and biochemical assays). Exploring the toxicity of pesticides, Bioanalytical and Statistics Core researchers found that several classes of insecticides, including pyrethroids, organochlorides, and diamides, elicited concentration-dependent increase ryanodine binding, suggesting possible neurotoxic effects (Pessah & Truong, 2019; Truong et al, 2019; Zheng et al, 2019). The Bioanalytical and Statistics Core demonstrate that structural changes in the ligand binding domain of the aryl hydrocarbon receptor (AhR) result in significant species differences in ligand response, suggesting difficulties when extrapolating from animal models to human (Faber et al, 2018). Using an ELISA assay developed with the Immunoassays for Human and Environmental Health Monitoring and Monitoring Endoplasmic Reticulum Stress Caused by Chronic Exposure to Chemicals Projects, the Core measured levels of soluble epoxide hydrolase (sEH) in peripheral blood mononuclear cells (PBMCs) of patients suffering from type 2 diabetes (Duflot et al 2019), linking the results in animal models from the Monitoring Endoplasmic Reticulum Stress Caused by Chronic Exposure to Chemicals project to patients’ health status. The Core provided support for the projects named above by characterizing epoxy-fatty acid degrading pathogenic microorganisms (deOliveira et al, 2019a & b; Vasylieva et al, 2019). Finally, the Core helped the Monitoring Endoplasmic Reticulum Stress Caused by Chronic Exposure to Chemicals Project obtain some inhibitors of enzymes involved in inflammation responses and pain perceptions (Burmistrov et al, 2019a & b; Codony et al, 2019; Hiesinger et al, 2019; Kramer et al, 2019; Lee et al, 2019). These tools are necessary to explore the mechanism by which xenobiotics affect health through epoxy-fatty acids regulated biology.