Superfund Research Program
Optimizing Bioremediation for Risk Reduction Using Integrated Bioassay, Non-Target Analysis and Genomic Mining Techniques
During this funding period, Project researchers worked with the Community Engagement Core, the Bioanalytical and Statistics Core, and the Yurok Tribal Environmental Program to collect, analyze and interpret the toxicological significance of water and sediment samples collected from multiple historical lumber processing sites (now abandoned) identified as being of concern to the community. The research team also developed and reported on novel methods to identify unknown endocrine disrupting or neurotoxic compounds in treated sewage sludge and house dust. The primary bottleneck in the biodegradation pathway for atrazine, a representative triazine herbicide and groundwater contaminant, was identified and enzyme engineering efforts have begun to improve expression of the required enzyme, thereby increasing the rate of atrazine hydrolysis and flux through the degradation pathway. Reactors to be used for experiments with organisms with engineered degradation pathways were retrofit to support automated operation during this period.