Title: Effects of salinity acclimation on the endocrine disruption and acute toxicity of bifenthrin in freshwater and euryhaline strains of Oncorhynchus mykiss.

Authors: Riar, Navneet; Crago, Jordan; Jiang, Weiying; Maryoung, Lindley A; Gan, Jay; Schlenk, Daniel

Published In Environ Toxicol Chem, (2013 Dec)

Abstract: The pyrethroid insecticide bifenthrin is frequently detected at ng/L concentrations in tributaries of the San Francisco Bay Delta. The estuary is also experiencing increasing salinity through climate change and water redirection. To evaluate the impacts of hypersaline conditions on bifenthrin toxicity in anadromous salmonids of the San Francisco Bay Delta (CA, USA), a 14-d laboratory exposure was performed using 2 strains of Oncorhynchus mykiss (rainbow trout and steelhead) acclimated to freshwater and to 8 g/L and 17 g/L salinity. The fish were then exposed to nominal concentrations of 0 µg/L, 0.1 µg/L, and 1.5 µg/L bifenthrin. Rainbow trout exhibited significant mortality following exposure to 1.5 µg/L (1.07 ± 0.35 µg/L measured) bifenthrin in freshwater. Elevated levels of Na⁺ /K⁺ adenosine triphosphatase α1A mRNA subunit expression was observed in the gill of rainbow trout acclimated to hypersaline conditions relative to freshwater animals. No significant difference was noted in Na⁺ /K⁺ adenosine triphosphatase subunit levels in brains of either strain in freshwater or hypersaline conditions. Likewise, significant differences were not observed in plasma vitellogenin or steroid hormone concentrations in either strain whether maintained in freshwater or saltwater. Saltwater acclimation significantly reduced nicotinamide adenine dinucleotide phosphate-catalyzed biotransformation of bifenthrin in liver microsomes of rainbow trout but not of steelhead. The present study showed that, relative to steelhead, rainbow trout have different responses to bifenthrin acute toxicity as well as different rates of hepatic bifenthrin biotransformation and regulation of Na⁺ /K⁺ adenosine triphosphatase subunits in gills. These data indicate that significant differences exist between the strains and that animal life history may have important effects on the susceptibility of each strain to environmental contaminants.

PubMed ID: 23983063

MeSH Terms: Adaptation, Physiological; Animals; Biotransformation; Brain/metabolism; California; Endocrine Disruptors/toxicity*; Estuaries; Fresh Water; Gills/metabolism; Insecticides/toxicity*; Isoenzymes/genetics; Isoenzymes/metabolism; Microsomes, Liver/metabolism; Oncorhynchus mykiss/metabolism*; Pyrethrins/toxicity*; RNA, Messenger/metabolism; Salinity; Sodium-Potassium-Exchanging ATPase/genetics; Sodium-Potassium-Exchanging ATPase/metabolism; Species Specificity; Vitellogenins/blood; Water Pollutants, Chemical/toxicity*