Title: Nanoglassified, optically-active monolayer films of gold nanoparticles for in situ orthogonal detection by localized surface plasmon resonance and surface-assisted laser desorption/ionization-MS.

Authors: Chen, Chih-Yuan; Hinman, Samuel S; Duan, Jicheng; Cheng, Quan

Published In Anal Chem, (2014 Dec 16)

Abstract: Localized surface plasmon resonance (LSPR) represents a sensitive and versatile method for detection of biomolecules in a label-free fashion, but identification of bound analytes can be challenging with LSPR alone, especially for samples in a complex medium. We report the fabrication of an optically active, plasmonic film of gold nanoparticles by using a self-assembly and calcination process, which offers orthogonal measurements enabling multifaceted characterization on the same surface with LSPR and surface-assisted laser desorption/ionization mass spectrometry. This proof-of-concept study involves plasmonic characterization of the fabricated nanofilm, real-time monitoring of vesicle-surface interactions toward formation of fluid lipid bilayer, and mass spectrometric analysis of peptides and cytochrome c digest. This multifunction-enabling surface material can yield complementary analytical information, providing new tools for comprehensive analysis of biomolecular samples.

PubMed ID: 25417963

MeSH Terms: Chemistry Techniques, Analytical/instrumentation*; Gold/chemistry*; Lasers*; Mass Spectrometry*; Metal Nanoparticles/chemistry*; Surface Plasmon Resonance*; Surface Properties