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Study Information
Study Attribute Value
CEBS Accession Number: 002-01134-0002-0000-3
Chemical Name: Perfluorobutane sulfonate (PFBS)
CASRN: 375-73-5
NTP Study Type: Genetic Toxicology - Micronucleus
NTP Study ID: G04006
Strain and Species: Harlan Sprague Dawley Rat
Study Result: Male: Negative; Female: Negative

Blood

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
2012-02-17 24 hour Male Flow Cytometry Gavage Gavage x 56, 28 day
  Dose
(mg/kg)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 0.46889553935009715		 Normochromatic Erythrocytes
Trend P: 0.9842781309195741
No. Examined Total MN Cells Percent PCE MN Cells
per 1000		 No. Examined Total MN Cells Percent NCE MN Cells
per 1000
Vehicle Control Deionized Water with 2% Tween 80 
 0         
 151 20000 20 1.0 1.0 2046151 491 99.0 0.2 
 0         
 152 20000 12 0.9 0.6 2180709 634 99.1 0.3 
 0         
 153 20000 10 0.9 0.5 2277962 1736 99.1 0.8 
 0         
 154 20000 6 1.1 0.3 1794126 424 98.9 0.2 
 0         
 155 20000 11 1.2 0.6 1634322 219 98.8 0.1
Average ± SEM   1.012 ± 0.063 0.590 ± 0.114   0.000 ± 0.000 0.333 ± 0.110
 
Test Chemical Perfluorobutane sulfonate (PFBS) 
31.3       
 161 20000 11 1.1 0.6 1824636 231 98.9 0.1 
31.3       
 162 20000 17 0.8 0.9 2617048 274 99.2 0.1 
31.3       
 163 20000 9 0.9 0.5 2314452 200 99.1 0.1 
31.3       
 164 20000 10 0.9 0.5 2237960 344 99.1 0.2 
31.3       
 165 20000 3 1.1 0.2 1861972 240 98.9 0.1
Average ± SEM   0.930 ± 0.063 0.500 ± 0.112   0.000 ± 0.000 0.120 ± 0.011
Pairwise P   0.617   1.000
 
Test Chemical Perfluorobutane sulfonate (PFBS) 
62.5       
 171 20000 14 1.1 0.7 1846462 145 98.9 0.1 
62.5       
 172 20000 12 0.7 0.6 2710593 168 99.3 0.1 
62.5       
 173 20000 9 0.9 0.5 2222899 308 99.1 0.1 
62.5       
 174 20000 14 0.8 0.7 2347891 379 99.2 0.2 
62.5       
 175 20000 13 1.0 0.7 1971264 426 99.0 0.2
Average ± SEM   0.909 ± 0.060 0.620 ± 0.046   0.000 ± 0.000 0.131 ± 0.028
Pairwise P   0.574   1.000
 
Test Chemical Perfluorobutane sulfonate (PFBS) 
125         
 181 20000 13 0.7 0.7 2946608 564 99.3 0.2 
125         
 182 20000 12 0.7 0.6 2915490 423 99.3 0.1 
125         
 183 20000 8 0.8 0.4 2562270 259 99.2 0.1 
125         
 184 20000 6 1.1 0.3 1860698 198 98.9 0.1 
125         
 185 19999 20 1.0 1.0 2066117 176 99.0 0.1
Average ± SEM   0.830 ± 0.078 0.590 ± 0.121   0.000 ± 0.000 0.126 ± 0.019
Pairwise P   0.608   1.000
 
Test Chemical Perfluorobutane sulfonate (PFBS) 
250         
 191 20000 9 0.7 0.5 2998524 332 99.3 0.1 
250         
 192 20000 14 0.4 0.7 5027898 577 99.6 0.1 
250         
 193 20000 9 0.7 0.5 2710601 316 99.3 0.1 
250         
 194 20000 19 0.2 1.0 8038573 1471 99.8 0.2 
250         
 196 19999 6 0.7 0.3 2741348 136 99.3 0.0
Average ± SEM   0.553 ± 0.098 0.570 ± 0.115   0.000 ± 0.000 0.115 ± 0.021
Pairwise P   0.626   1.000
 

Blood

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
2012-02-18 24 hour Female Flow Cytometry Gavage Gavage x 56, 28 day
  Dose
(mg/kg)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 0.1539614421866628		 Normochromatic Erythrocytes
Trend P: 0.647289124895783
No. Examined Total MN Cells Percent PCE MN Cells
per 1000		 No. Examined Total MN Cells Percent NCE MN Cells
per 1000
Vehicle Control Deionized Water with 2% Tween 80 
 0         
 211 20000 17 0.8 0.9 2505082 146 99.2 0.1 
 0         
 212 20000 14 1.7 0.7 1141130 55 98.3 0.0 
 0         
 213 20000 9 1.1 0.5 1881074 188 98.9 0.1 
 0         
 214 20000 17 1.1 0.9 1751868 110 98.9 0.1 
 0         
 215 20000 5 1.4 0.3 1378705 33 98.6 0.0
Average ± SEM   1.225 ± 0.161 0.620 ± 0.118   0.000 ± 0.000 0.059 ± 0.012
 
Test Chemical Perfluorobutane sulfonate (PFBS) 
31.3       
 221 20000 11 0.7 0.6 2889171 186 99.3 0.1 
31.3       
 222 20000 17 0.7 0.9 2879473 149 99.3 0.1 
31.3       
 223 19999 8 0.9 0.4 2228058 308 99.1 0.1 
31.3       
 224 20000 7 0.5 0.4 3761326 249 99.5 0.1 
31.3       
 225 20000 8 1.1 0.4 1786958 196 98.9 0.1
Average ± SEM   0.781 ± 0.100 0.510 ± 0.091   0.000 ± 0.000 0.086 ± 0.016
Pairwise P   0.630   0.159
 
Test Chemical Perfluorobutane sulfonate (PFBS) 
62.5       
 231 20000 22 0.7 1.1 2860998 183 99.3 0.1 
62.5       
 232 20000 19 0.8 1.0 2500955 263 99.2 0.1 
62.5       
 233 20000 3 0.9 0.2 2159474 269 99.1 0.1 
62.5       
 234 20000 12 0.7 0.6 2750663 80 99.3 0.0 
62.5       
 235 20000 7 0.9 0.4 2290026 174 99.1 0.1
Average ± SEM   0.799 ± 0.042 0.630 ± 0.178   0.000 ± 0.000 0.080 ± 0.017
Pairwise P   0.558   0.192
 
Test Chemical Perfluorobutane sulfonate (PFBS) 
125         
 241 20000 19 0.6 1.0 3526417 338 99.4 0.1 
125         
 242 20000 16 0.4 0.8 5070494 620 99.6 0.1 
125         
 243 20000 16 1.1 0.8 1884725 184 98.9 0.1 
125         
 244 20000 12 0.5 0.6 3662669 200 99.5 0.1 
125         
 245 20000 11 1.0 0.6 2068797 136 99.0 0.1
Average ± SEM   0.701 ± 0.128 0.740 ± 0.073   0.000 ± 0.000 0.087 ± 0.012
Pairwise P   0.349   0.206
 
Test Chemical Perfluorobutane sulfonate (PFBS) 
250         
 251 20000 17 0.7 0.9 2862528 222 99.3 0.1 
250         
 252 20000 8 0.4 0.4 4879709 168 99.6 0.0 
250         
 253 20000 14 0.9 0.7 2278745 120 99.1 0.1 
250         
 254 20001 17 0.4 0.8 4620940 212 99.6 0.0 
250         
 256 20000 14 0.4 0.7 5529934 530 99.6 0.1
Average ± SEM   0.553 ± 0.098 0.700 ± 0.082   0.000 ± 0.000 0.061 ± 0.011
Pairwise P   0.362   0.213