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Study Information
Study Attribute Value
CEBS Accession Number: 002-01369-0001-0000-2
Chemical Name: 2-Nitrodiphenylamine
CASRN: 119-75-5
NTP Study Type: Genetic Toxicology - Bacterial Mutagenicity
NTP Study ID: 731425
Vehicle Control: Dimethyl Sulfoxide
Study Result: Negative

Table Instructions and Notes:
You are currently viewing the results for this Salmonella study's detailed data.
Individual strain data is presented as mean ± standard error.
Abbreviations are noted at bottom of page.
Trial summary calls are shown in parentheses.
Standard NTP Protocol

Strain: TA100
Dose No Activation
(Negative) 		No Activation
(Negative) 		30% RLI
(Negative) 		30% HLI
(Negative) 		10% RLI
(Negative) 		10% HLI
(Negative)
Protocol Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 114 4.9 134 9.3 149 12.2 113 1.2 148 6.4 134 13.8 
 0.3       
 96 6 122 9.3 
1         
 110 13.7 123 3.5 
3         
 98 5.5 100 9.9 138 1.9 
10         
 96 3.5 94 7.6 114 4.6 110 15.1 137 14.2 132 8.3 
16         
 104 2.7 
33         
 28 s 6.4 129 18.4 93 5.9 136 3 124 18.4 
100         
 116 8.2 96 3 127 7.8 134 8.8 
333         
 94 10.2 59 29.8 93 9.7 101 8.2 
666         
 21 s 4.8 83 s 3.9 
1000         
 0 s 0
Positive Control 330 14.3 342 .6 483 4.9 238 17.8 316 2.3 337 8.4

Strain: TA1535
Dose No Activation
(Negative) 		No Activation
(Negative) 		30% RLI
(Negative) 		30% HLI
(Negative) 		10% RLI
(Negative) 		10% HLI
(Negative)
Protocol Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 24 1.7 10 .6 11 2.6 7 2.2 9 1.3 11 2.7 
 0.3       
 22 .9 11 1.9 
1         
 16 1.2 7 .6 
3         
 17 1.8 7 1.5 12 3 
10         
 18 2.2 10 2.2 10 1.9 7 .6 10 4.2 8 .3 
16         
 4 s .9 
33         
 4 s 2.1 12 .6 6 .6 13 .6 12 5 
100         
 13 2.3 6 2.1 8 1.9 9 1.3 
333         
 8 2.6 5 1.5 9 1.3 8 1.3 
666         
 1 s .6 0 s .3 
1000         
 0 0
Positive Control 232 14.9 104 4.4 316 23 138 21.4 100 6.5 138 12

Strain: TA97
Dose No Activation
(Negative) 		No Activation
(Negative) 		5% HLI
(Negative) 		30% RLI
(Negative) 		30% HLI
(Equivocal) 		30% HLI
(Negative) 		10% RLI
(Negative) 		10% HLI
(Equivocal) 		10% HLI
(Negative)
Protocol Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 150 6.7 145 7.9 182 11.2 177 14.5 120 12.2 182 8.4 165 11 152 1 165 7.2 
 0.3       
 155 7.4 145 14.2 
1         
 143 4 121 10.3 
3         
 143 10.3 124 11.7 176 14 
10         
 134 4.3 128 15.8 182 2 166 10.3 187 6.2 211 5.2 195 12.6 191 4.5 168 8.2 
16         
 95 10.3 
33         
 125 7.9 211 14.2 140 15.1 193 5 212 5 159 4.3 196 5.5 156 7.2 
100         
 201 3.5 109 56.3 188 1.5 200 11.7 175 12.2 177 7.6 173 13.8 
333         
 119 11.9 85 10.2 162 7 176 1.2 45 15.5 87 7 155 7.5 
666         
 7 s .9 11 3.1 51 s 17.2 47 s 5.4 16 s 4.5 
1000         
 3 1.2
Positive Control 550 19.9 823 89.5 596 18 471 14.4 324 12.5 348 8.2 530 20 595 60.6 398 16.4

Strain: TA98
Dose No Activation
(Negative) 		No Activation
(Negative) 		30% RLI
(Negative) 		30% HLI
(Negative) 		10% RLI
(Negative) 		10% HLI
(Negative)
Protocol Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 18 2.1 21 1.9 20 2 30 3.2 27 2.9 27 3 
 0.3       
 19 3.8 14 1.5 
1         
 13 4.3 15 2 
3         
 14 3.7 13 4 30 .9 
10         
 17 .7 14 2.3 24 4 32 3 33 4.2 30 2.3 
16         
 14 1 
33         
 12 2.7 22 2.1 27 1.7 28 5.2 43 2.3 
100         
 18 .9 29 4.5 24 4 32 2.9 
333         
 21 1.9 30 4.4 24 6.9 28 .3 
666         
 15 2.8 26 .9 
1000         
 20 2.4
Positive Control 394 16.8 493 7.5 173 10.4 86 2.5 272 7.4 769 6.9

Abbreviations:
RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9
s = Slight Toxicity; p = Precipitate; x = Slight Toxicity and Precipitate; t = Toxic;