| Study Attribute | Value |
|---|---|
| CEBS Accession Number: | 002-01525-0003-0000-8 |
| Chemical Name: | 5,5-Diphenylhydantoin (phenytoin) |
| CASRN: | 57-41-0 |
| NTP Study Type: | Genetic Toxicology - Micronucleus |
| NTP Study ID: | 110489 |
| Strain and Species: | BALB/C Mouse |
| Study Result: | Male: Negative |
Bone Marrow
| Start Date | Sample Collection Time | Sex | Methodology Used | Route | Dosing Regimen |
|---|---|---|---|---|---|
| 1990-09-04 | 24 hour | Male | Slide Scoring | Intraperitoneal Injection | Intraperitoneal Injection x 1, 24 hour |
| Dose (mg/kg) |
Animal Number |
Polychromatic Erythrocytes Trend P: 0.211 |
|||||
|---|---|---|---|---|---|---|---|
| No. Examined | Total MN Cells | Percent PCE | MN Cells per 1000 |
||||
| Vehicle Control | Naoh | 0 |
398 | 2000 | 6 | 51.0 | 3.0 |
0 |
399 | 2000 | 8 | 62.5 | 4.0 | ||
0 |
400 | 2000 | 5 | 48.5 | 2.5 | ||
0 |
401 | 2000 | 5 | 53.0 | 2.5 | ||
0 |
402 | 2000 | 7 | 57.0 | 3.5 | ||
| Average ± SEM | 54.400 ± 2.460 | 3.100 ± 0.290 | |||||
| Test Chemical | 5,5-Diphenylhydantoin (phenytoin) | 0.1 |
358 | 2000 | 13 | 54.5 | 6.5 |
0.1 |
359 | 2000 | 2 | 60.5 | 1.0 | ||
0.1 |
360 | 2000 | 7 | 52.5 | 3.5 | ||
0.1 |
361 | 2000 | 4 | 45.5 | 2.0 | ||
0.1 |
362 | 2000 | 0 | 48.5 | 0.0 | ||
| Average ± SEM | 52.300 ± 2.580 | 2.600 ± 1.130 | Pairwise P | 0.678 | |||
| Test Chemical | 5,5-Diphenylhydantoin (phenytoin) | 0.5 |
363 | 2000 | 8 | 38.5 | 4.0 |
0.5 |
364 | 2000 | 8 | 42.5 | 4.0 | ||
0.5 |
365 | 2000 | 6 | 46.5 | 3.0 | ||
0.5 |
366 | 2000 | 8 | 52.5 | 4.0 | ||
0.5 |
367 | 2000 | 2 | 65.0 | 1.0 | ||
0.5 |
388 | 2000 | 11 | 58.5 | 5.5 | ||
0.5 |
389 | 2000 | 9 | 71.0 | 4.5 | ||
0.5 |
390 | 2000 | 5 | 40.5 | 2.5 | ||
0.5 |
391 | 2000 | 6 | 60.5 | 3.0 | ||
0.5 |
392 | 2000 | 8 | 51.0 | 4.0 | ||
| Average ± SEM | 49.000 ± 4.620 | 3.200 ± 0.580 | Pairwise P | 0.465 | |||
| Test Chemical | 5,5-Diphenylhydantoin (phenytoin) | 1 |
368 | 2000 | 6 | 34.0 | 3.0 |
1 |
369 | 2000 | 3 | 48.5 | 1.5 | ||
1 |
370 | 2000 | 8 | 54.0 | 4.0 | ||
1 |
371 | 2000 | 14 | 41.0 | 7.0 | ||
1 |
372 | 2000 | 10 | 46.5 | 5.0 | ||
| Average ± SEM | 44.800 ± 3.410 | 4.100 ± 0.930 | Pairwise P | 0.205 | |||
| Test Chemical | 5,5-Diphenylhydantoin (phenytoin) | 5 |
373 | 2000 | 2 | 44.5 | 1.0 |
5 |
374 | 2000 | 6 | 57.0 | 3.0 | ||
5 |
375 | 2000 | 10 | 66.0 | 5.0 | ||
5 |
376 | 2000 | 10 | 41.0 | 5.0 | ||
5 |
377 | 2000 | 2 | 16.0 | 1.0 | ||
5 |
393 | 2000 | 14 | 44.5 | 7.0 | ||
5 |
394 | 2000 | 20 | 40.0 | 10.0 | ||
5 |
395 | 2000 | 10 | 52.5 | 5.0 | ||
5 |
396 | 2000 | 20 | 58.5 | 10.0 | ||
5 |
397 | 2000 | 32 | 54.0 | 16.0 | ||
| Average ± SEM | 44.900 ± 8.490 | 3.000 ± 0.890 | Pairwise P | 0.536 | |||
| Test Chemical | 5,5-Diphenylhydantoin (phenytoin) | 10 |
378 | 2000 | 9 | 50.5 | 4.5 |
10 |
379 | 2000 | 5 | 59.0 | 2.5 | ||
10 |
380 | 2000 | 8 | 33.5 | 4.0 | ||
10 |
381 | 2000 | 10 | 45.0 | 5.0 | ||
10 |
382 | 2000 | 1 | 54.0 | 0.5 | ||
| Average ± SEM | 48.400 ± 4.370 | 3.300 ± 0.820 | Pairwise P | 0.431 | |||
| Test Chemical | 5,5-Diphenylhydantoin (phenytoin) | 20 |
383 | 2000 | 10 | 34.0 | 5.0 |
20 |
384 | 2000 | 10 | 41.0 | 5.0 | ||
20 |
385 | 2000 | 11 | 39.0 | 5.5 | ||
20 |
386 | 2000 | 4 | 58.0 | 2.0 | ||
20 |
387 | 2000 | 5 | 47.0 | 2.5 | ||
| Average ± SEM | 43.800 ± 4.120 | 4.000 ± 0.720 | Pairwise P | 0.228 | |||
| Positive Control | Mitomycin-C | 0.5 |
363 | 2000 | 8 | 38.5 | 4.0 |
0.5 |
364 | 2000 | 8 | 42.5 | 4.0 | ||
0.5 |
365 | 2000 | 6 | 46.5 | 3.0 | ||
0.5 |
366 | 2000 | 8 | 52.5 | 4.0 | ||
0.5 |
367 | 2000 | 2 | 65.0 | 1.0 | ||
0.5 |
388 | 2000 | 11 | 58.5 | 5.5 | ||
0.5 |
389 | 2000 | 9 | 71.0 | 4.5 | ||
0.5 |
390 | 2000 | 5 | 40.5 | 2.5 | ||
0.5 |
391 | 2000 | 6 | 60.5 | 3.0 | ||
0.5 |
392 | 2000 | 8 | 51.0 | 4.0 | ||
| Average ± SEM | 56.300 ± 5.080 | 3.900 ± 0.530 | Pairwise P | 0.169 | |||
| Positive Control | Mitomycin-C | 5 |
373 | 2000 | 2 | 44.5 | 1.0 |
5 |
374 | 2000 | 6 | 57.0 | 3.0 | ||
5 |
375 | 2000 | 10 | 66.0 | 5.0 | ||
5 |
376 | 2000 | 10 | 41.0 | 5.0 | ||
5 |
377 | 2000 | 2 | 16.0 | 1.0 | ||
5 |
393 | 2000 | 14 | 44.5 | 7.0 | ||
5 |
394 | 2000 | 20 | 40.0 | 10.0 | ||
5 |
395 | 2000 | 10 | 52.5 | 5.0 | ||
5 |
396 | 2000 | 20 | 58.5 | 10.0 | ||
5 |
397 | 2000 | 32 | 54.0 | 16.0 | ||
| Average ± SEM | 49.900 ± 3.350 | 9.600 ± 1.860 | Pairwise P | Value less than 0.0001 | |||