Genetic Toxicology - Bacterial Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01525-0006-0000-1
Chemical Name:	5,5-Diphenylhydantoin (phenytoin)
CASRN:	57-41-0
NTP Study Type:	Genetic Toxicology - Bacterial Mutagenicity
NTP Study ID:	599479
Vehicle Control:	Dimethyl Sulfoxide
Study Result:	Negative

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Table Instructions and Notes:
You are currently viewing the results for this Salmonella study's detailed data.
Individual strain data is presented as mean ± standard error.
Abbreviations are noted at bottom of page.
Trial summary calls are shown in parentheses.
Standard NTP Protocol

Strain: TA100
Dose	No Activation (Negative)		No Activation (Negative)		10% RLI (Negative)		10% RLI (Negative)		10% HLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	106	8.7	94	.7	118	9.2	98	7.2	114	1.7	101	2.4
100	105	2.4	87	3.8	94	8.4	97	3.8	110	6.1	103	3.3
333	103	5	89	7.8	101	4.7	108	4.7	108	9.7	92	3.3
1000	81	3.3	84	3.8	93	5	107	8.4	99	8.7	102	3.2
3333	79 p	2.9	86 p	3.2	96 p	12.8	100 p	3.3	90 p	3.8	98 p	.6
10000	73 p	5.6	63 p	6	94 p	3.1	95 p	5.5	105 p	2.9	84 p	3.5
Positive Control	1417	96.6	2161	64.3	1337	70	632	61.1	2384	21.1	838	80.8

Strain: TA1535
Dose	No Activation (Negative)		No Activation (Negative)		10% RLI (Negative)		10% RLI (Negative)		10% HLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	15	1.5	20	1.5	9	1.3	11	1.9	10	4	11	1.5
100	14	3.5	24	2.6	11	2.4	8	2.5	8	2.1	11	2.3
333	14	1	23	2.3	7	1.3	9	2.3	9	1.5	11	2.7
1000	21	.9	24	2.8	7	.9	6	2.1	10	.9	8	2.9
3333	18 p	.3	18 p	.3	5 p	1.3	10 p	.9	10 p	.6	8 p	2.3
10000	15 p	1.2	12 p	2.1	6 p	.3	9 p	1.5	5 p	.3	9 s	1.5
Positive Control	1068	11.4	1496	75.6	84	2.9	56	7.2	117	13.5	62	12.2

Strain: TA1537
Dose	No Activation (Negative)		No Activation (Negative)		10% RLI (Negative)		10% RLI (Negative)		10% HLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	7	2	4	1.2	6	2.3	9	1.3	8	2.1	7	2.1
100	5	.3	6	2.5	6	1.5	6	.6	7	.7	5	2
333	4	1.5	6	2.2	7	1.2	7	1.2	5	.6	7	.3
1000	7	2.6	4	.9	4	.3	5	.6	6	.9	4	1.2
3333	5 p	.6	4 p	.9	7 p	1.5	5 p	.9	5 p	1	4 p	.6
10000	3 p	.9	1 p	.7	5 p	1.7	4 p	.3	3 p	.7	3 p	1.2
Positive Control	936	151.4	287	6.4	60	4.7	48	10.4	135	18.2	92	10.8

Strain: TA98
Dose	No Activation (Negative)		No Activation (Negative)		10% RLI (Negative)		10% RLI (Negative)		10% HLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	15	1.5	10	2.1	24	3	20	5.2	24	2.2	25	2
100	23	2	14	.6	16	3.4	27	2.3	24	1.9	20	1.5
333	19	1.7	13	2.3	19	1.7	24	.9	27	2.1	26	1.9
1000	14	.3	17	2.3	25	4.1	22	.3	22	1.9	19	.9
3333	17 p	.7	13 p	.7	21 p	2	20	1.9	23 p	4.5	22 p	1.2
10000	10 p	2.9	8 p	.3	15 p	2.9	21	2.5	16 p	4.2	18 p	2.2
Positive Control	1449	94.2	1812	30.2	1029	83	361	22	2027	72.1	428	28.5

Abbreviations:
RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9
s = Slight Toxicity; p = Precipitate; x = Slight Toxicity and Precipitate; t = Toxic;