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Study Information
Study Attribute Value
CEBS Accession Number: 002-01536-0005-0000-2
Chemical Name: 5-Azacytidine
CASRN: 320-67-2
NTP Study Type: Genetic Toxicology - Bacterial Mutagenicity
NTP Study ID: A21960
Vehicle Control: Dimethyl Sulfoxide
Study Result: Positive

Table Instructions and Notes:
You are currently viewing the results for this Salmonella study's detailed data.
Individual strain data is presented as mean ± standard error.
Abbreviations are noted at bottom of page.
Trial summary calls are shown in parentheses.
Standard NTP Protocol

Strain: TA100
Dose No Activation
(Negative) 		30% RLI
(Equivocal) 		30% RLI
(Negative) 		30% HLI
(Equivocal) 		30% HLI
(Negative)
Protocol Preincubation Preincubation Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 111 3.8 127 1 110 3.4 109 12.1 107 1.8 
 0.3       
 93 3.2 110 3 104 3.8 
 0.6       
 107 2.5 113 4.7 
1         
 115 7.7 168 5 100 4.4 161 9.2 106 8.6 
1.6       
 96 4.6 98 5 
3         
 115 7.2 184 14.4 85 1 181 3.5 91 4 
10         
 125 .3 115 3.9 126 7.8 
16         
 101 s 5 
33         
 104 2.7 144 5.1 
100         
 102 s 7.6 96 s 3.6
Positive Control 914 8 792 15.9 371 11.5 603 25.1 486 37.9

Strain: TA102
Dose No Activation
(Positive) 		No Activation
(Positive) 		30% RLI
(Positive) 		30% RLI
(Positive) 		30% HLI
(Positive) 		30% HLI
(Positive)
Protocol Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 200 16.3 205 7.8 233 24.1 276 16 165 19.7 285 5 
 0.01      
 220 8.8 
 0.03      
 249 7.8 324 13.9 
 0.1       
 295 13.4 365 4.2 362 15.4 
 0.3       
 800 8.7 489 22.2 663 27.6 640 92.1 
1         
 1487 37.8 810 38.8 752 11.6 1213 167.6 390 22.2 1096 55.8 
1.6       
 1295 30.7 
3         
 0 x 0 1333 26.8 1425 16.6 694 15.5 1560 28.9 
10         
 0 x 0 0 x 0 0 x 0 
16         
 0 x 0 
33         
 0 x 0 0 x 0 
100         
 0 x 0 0 x 0
Positive Control 937 7.1 662 38.2 551 19.1 704 17.8 728 5.9 901 35.1

Strain: TA104
Dose No Activation
(Positive) 		No Activation
(Positive) 		30% RLI
(Equivocal) 		30% RLI
(Weakly Positive) 		30% HLI
(Positive) 		30% HLI
(Positive)
Protocol Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 244 21.9 243 13 370 19.3 317 20.3 310 11.1 372 18.3 
 0.03      
 307 14.7 
 0.1       
 339 3.6 
 0.3       
 284 8.5 251 2.7 388 10.1 406 31.2 
1         
 330 8.7 300 18.5 510 5.8 436 9.6 475 13.8 502 17.9 
3         
 339 9.9 376 10.7 502 13.3 448 14.2 527 3.2 518 3.3 
10         
 376 15.7 417 11.1 330 15 535 2.3 536 11 
16         
 490 13 480 12.1 
33         
 328 25.7 692 42.1 884 12.7 
100         
 216 .7 233 61.7
Positive Control 660 10.7 565 6.4 575 19.7 828 8.5 647 .9 828 10.1

Strain: TA1535
Dose No Activation
(Positive) 		No Activation
(Weakly Positive) 		30% RLI
(Positive) 		30% RLI
(Positive) 		30% HLI
(Positive) 		30% HLI
(Positive)
Protocol Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 11 1.7 11 .9 17 2.4 15 .3 15 .9 13 .9 
 0.3       
 12 1.8 10 .6 16 5 21 3.2 
1         
 19 .9 12 1.5 30 4 17 2 30 .9 23 2.3 
3         
 26 3.4 15 .9 37 4 36 1.5 44 2.3 34 5.2 
10         
 25 2.9 18 .3 49 3 92 6 45 3.4 102 7.6 
33         
 29 5 22 1.3 84 2.6 100 3.9 80 5.2 141 9.1 
100         
 18 4.4 16 s 3.5
Positive Control 851 7.9 399 16.5 222 6.6 190 3.5 321 15.1 150 14.8

Strain: TA97
Dose No Activation
(Negative) 		30% RLI
(Negative) 		30% HLI
(Negative)
Protocol Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 123 4.6 132 11.7 132 3.9 
 0.3       
 120 9.7 
1         
 142 8.4 161 10.5 135 3.2 
3         
 106 2.3 177 2.4 173 8.5 
10         
 131 22.7 107 4.4 109 4.3 
33         
 112 9.4 152 7.8 146 9.8 
100         
 151 7 144 14.3
Positive Control 426 13 435 23.4 481 18.2

Strain: TA98
Dose No Activation
(Negative) 		30% RLI
(Negative) 		30% HLI
(Negative)
Protocol Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 13 2 12 1.8 15 2.5 
 0.3       
 13 2.1 
1         
 13 2.2 15 1.3 20 1.5 
3         
 11 1 14 2.6 18 1.7 
10         
 10 .6 11 1.2 14 1.9 
16         
 6 s .9 
33         
 12 .9 15 1 
100         
 4 s .6 5 s 1
Positive Control 328 28.1 669 17 291 13.3

Abbreviations:
RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9
s = Slight Toxicity; p = Precipitate; x = Slight Toxicity and Precipitate; t = Toxic;