Genetic Toxicology - Bacterial Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01695-0001-0000-4
Chemical Name:	Benzidine, 2,2'-disulfonic acid
CASRN:	117-61-3
NTP Study Type:	Genetic Toxicology - Bacterial Mutagenicity
NTP Study ID:	619780
Vehicle Control:	Dimethyl Sulfoxide
Study Result:	Negative

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Table Instructions and Notes:
You are currently viewing the results for this Salmonella study's detailed data.
Individual strain data is presented as mean ± standard error.
Abbreviations are noted at bottom of page.
Trial summary calls are shown in parentheses.
Standard NTP Protocol

Strain: TA100
Dose	No Activation (Negative)		No Activation (Negative)		30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	127	8.3	127	14.7	155	7.7	167	5	133	5.2	145	2.4
100	149	13.5	149	1.2	150	10.8	147	11.3	162	8.4	157	5.3
333	153	3.5	121	18.8	151	9.9	160	5.9	149	.6	156	6.7
1000	125	1.2	137	15.9	150	4.1	156	4.3	136	4.7	151	3.5
3333	141	6.8	133	7.2	144	10.8	141	2	134	3.5	135	5
10000	154	2	139	7.5	149	6.7	153	8.3	131	9.9	132	5.5
Positive Control	876	24	1009	15.9	501	36.2	723	5.8	288	3.6	400	48.5

Strain: TA1535
Dose	No Activation (Negative)		No Activation (Negative)		30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	10	1.5	12	2	15	1.5	12	1.8	17	1.5	16	3.6
100	10	1.9	19	1.5	18	2.8	15	.6	15	1.2	13	3.1
333	12	3.2	13	2.3	17	1.5	13	3.2	13	2.6	12	2.3
1000	12	1.9	16	.6	12	1.5	9	2	16	1.5	14	2.8
3333	14	0	15	1.3	15	1	11	2.4	12	3	13	2.2
10000	10	2.6	12	2.6	14	1.2	14	1.2	13	.6	10	3.1
Positive Control	949	5.6	941	34.4	112	16.9	333	10.4	86	4.6	100	2.1

Strain: TA1538
Dose	30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	19	3.3	29	.3	21	3.9	18	1.8
100	18	2	23	1.3	20	1.9	18	1.8
333	16	1.2	22	3.5	14	1.5	18	5.8
1000	16	2.6	18	3	19	3.4	18	2
3333	23	3.7	21	3.8	15	1.5	21	3.8
10000	22	2.7	13	3.5	13	4.1	22	2.2
Positive Control	523	17.2	705	16.4	86	3.7	89	2.9

Strain: TA97
Dose	No Activation (Negative)		No Activation (Negative)		30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	139	5.7	122	7.1	159	16.9	125	7.8	153	11.1	147	2.6
100	115	3.5	131	10.8	161	5.4	121	1.2	155	16.5	153	11.3
333	118	16.5	145	10.7	165	11.3	124	5.5	166	15.7	152	11.3
1000	116	2.8	142	6.9	170	12.2	167	19.3	159	7.5	139	15.3
3333	121	3	140	6.4	170	24.2	150	12.1	148	6.7	148	6.4
10000	150	9	141	12.8	152	16.3	156	8	145	13.7	138	8.4
Positive Control	328	11.9	300	19.2	401	36.4	523	51.7	363	17.5	333	16.1

Strain: TA98
Dose	No Activation (Negative)		No Activation (Negative)		30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	18	.7	24	2.8	21	3.1	21	.3	27	.6	29	.9
100	17	.9	21	1.9	22	1.7	26	1.5	26	.7	26	.9
333	15	.9	19	2.5	22	3.7	26	1.8	23	2.6	22	2.8
1000	16	1.2	18	3.5	23	1.2	21	3.5	22	2.3	26	6.7
3333	16	.9	22	3.1	24	.9	23	.3	22	2.2	25	2.3
10000	17	1.2	19	3.2	18	1.9	23	4.7	18	1.2	24	2.6
Positive Control	349	5.1	313	15.3	163	4.9	232	27.8	108	4.5	134	7.5

Abbreviations:
RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9
s = Slight Toxicity; p = Precipitate; x = Slight Toxicity and Precipitate; t = Toxic;