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Study Information
Study Attribute Value
CEBS Accession Number: 002-02128-0001-0000-6
Chemical Name: Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE)
CASRN: 54464-57-2
NTP Study Type: Genetic Toxicology - Micronucleus
NTP Study ID: F28470
Strain and Species: F344/NTac Rat
Study Result: Male: Negative; Female: Negative

Blood

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
2006-03-13 24 hour Male Flow Cytometry Dermal Dermal x 91, 13 week
  Dose
(%)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 0.045		 Normochromatic Erythrocytes
Trend P: 0.963
No. Examined Total MN Cells Percent PCE MN Cells
per 1000		 No. Examined Total MN Cells Percent NCE MN Cells
per 1000
Vehicle Control 95% Ethanol 
 0         
 11 20000 11 1.1 0.6 1784456 138 98.9 0.1 
 0         
 12 20000 4 1.0 0.2 1937347 131 99.0 0.1 
 0         
 13 20000 6 1.4 0.3 1388135 165 98.6 0.1 
 0         
 14 20000 9 1.2 0.5 1702730 114 98.8 0.1 
 0         
 15 20000 8 1.2 0.4 1614279 143 98.8 0.1
Average ± SEM   1.201 ± 0.069 0.380 ± 0.060   0.000 ± 0.000 0.084 ± 0.010
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
6.25      
 21 20000 10 1.1 0.5 1825412 165 98.9 0.1 
6.25      
 22 20000 4 1.2 0.2 1685102 95 98.8 0.1 
6.25      
 23 20000 6 1.2 0.3 1662785 75 98.8 0.0 
6.25      
 24 20000 13 1.1 0.7 1759616 187 98.9 0.1 
6.25      
 25 20000 10 1.2 0.5 1591842 93 98.8 0.1
Average ± SEM   1.176 ± 0.028 0.430 ± 0.080   0.000 ± 0.000 0.071 ± 0.012
Pairwise P   0.431   0.980
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
12.5       
 31 20000 7 1.2 0.4 1632769 80 98.8 0.0 
12.5       
 32 20000 8 1.1 0.4 1753484 42 98.9 0.0 
12.5       
 33 20000 7 1.2 0.4 1663938 85 98.8 0.1 
12.5       
 34 20000 9 1.2 0.5 1624141 54 98.8 0.0 
12.5       
 35 20000 5 1.3 0.3 1491369 59 98.7 0.0
Average ± SEM   1.228 ± 0.032 0.360 ± 0.033   0.000 ± 0.000 0.039 ± 0.005
Pairwise P   0.509   0.993
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
25         
 41 20000 13 1.2 0.7 1584518 80 98.8 0.1 
25         
 42 20000 14 1.4 0.7 1377155 62 98.6 0.0 
25         
 43 20000 12 1.3 0.6 1496913 86 98.7 0.1 
25         
 44 20000 9 1.0 0.5 1941546 73 99.0 0.0 
25         
 45 20000 17 1.4 0.9 1444483 65 98.6 0.0
Average ± SEM   1.293 ± 0.073 0.650 ± 0.065   0.000 ± 0.000 0.047 ± 0.003
Pairwise P   0.021   0.996
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
50         
 51 20000 10 0.9 0.5 2137044 140 99.1 0.1 
50         
 52 20000 7 0.9 0.4 2120665 115 99.1 0.1 
50         
 53 20000 9 1.0 0.5 1947368 137 99.0 0.1 
50         
 54 20000 14 1.3 0.7 1568957 41 98.7 0.0 
50         
 55 20000 10 0.8 0.5 2420100 128 99.2 0.1
Average ± SEM   1.001 ± 0.075 0.500 ± 0.057   0.000 ± 0.000 0.054 ± 0.008
Pairwise P   0.022   0.997
 

Blood

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
2006-03-13 24 hour Male Flow Cytometry Dermal Dermal x 91, 13 week
  Dose
(%)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 		Normochromatic Erythrocytes
Trend P:
No. Examined Total MN Cells Percent PCE MN Cells
per 1000		 No. Examined Total MN Cells Percent NCE MN Cells
per 1000
Untreated Control 
 0         
 1 20000 8 1.01 0.40 1956627 267 98.99 0.1 
 0         
 2 20000 10 1.06 0.50 1869281 182 98.94 0.1 
 0         
 3 20000 8 1.22 0.40 1613159 113 98.78 0.1 
 0         
 4 20000 7 1.25 0.35 1580038 155 98.75 0.1 
 0         
 5 20000 5 1.01 0.25 1953964 131 98.99 0.1
Average ± SEM   1.124 ± 0.053 0.380 ± 0.041   0.000 ± 0.000 0.094 ± 0.013
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
100         
 61 20000 9 1.0 0.5 1962099 86 99.0 0.0 
100         
 62 20001 4 1.2 0.2 1690997 66 98.8 0.0 
100         
 63 20000 5 1.3 0.3 1470092 71 98.7 0.0 
100         
 64 20000 14 1.0 0.7 1972356 49 99.0 0.0 
100         
 65 20000 11 1.0 0.6 2043254 50 99.0 0.0
Average ± SEM   1.111 ± 0.072 0.430 ± 0.093   0.000 ± 0.000 0.036 ± 0.005
Pairwise P   0.320   0.969
 


Blood

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
2006-03-14 24 hour Female Flow Cytometry Dermal Dermal x 91, 13 week
  Dose
(%)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 0.454		 Normochromatic Erythrocytes
Trend P: 0.910
No. Examined Total MN Cells Percent PCE MN Cells
per 1000		 No. Examined Total MN Cells Percent NCE MN Cells
per 1000
Vehicle Control 95% Ethanol 
 0         
 81 20000 21 0.9 1.1 2109575 228 99.1 0.1 
 0         
 82 20000 10 0.6 0.5 3208723 118 99.4 0.0 
 0         
 83 20000 14 1.0 0.7 2014794 341 99.0 0.2 
 0         
 84 20000 3 0.7 0.2 2840087 100 99.3 0.0 
 0         
 85 20000 10 0.7 0.5 2879094 101 99.3 0.0
Average ± SEM   0.793 ± 0.074 0.580 ± 0.147   0.000 ± 0.000 0.077 ± 0.027
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
6.25      
 91 20000 5 1.0 0.3 1946977 97 99.0 0.0 
6.25      
 92 20000 9 0.5 0.5 3672156 126 99.5 0.0 
6.25      
 93 20000 10 1.1 0.5 1721541 72 98.9 0.0 
6.25      
 94 20000 11 0.6 0.6 3492602 166 99.4 0.0 
6.25      
 95 20000 5 0.9 0.3 2289431 50 99.1 0.0
Average ± SEM   0.836 ± 0.122 0.400 ± 0.063   0.000 ± 0.000 0.039 ± 0.005
Pairwise P   0.690   1.000
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
12.5       
 101 20000 9 1.0 0.5 1978787 61 99.0 0.0 
12.5       
 102 20000 3 1.0 0.2 1916659 80 99.0 0.0 
12.5       
 103 20000 8 0.9 0.4 2112908 167 99.1 0.1 
12.5       
 104 20000 27 0.9 1.4 2243449 236 99.1 0.1 
12.5       
 105 20000 8 1.5 0.4 1352107 43 98.5 0.0
Average ± SEM   1.074 ± 0.105 0.550 ± 0.207   0.000 ± 0.000 0.058 ± 0.015
Pairwise P   0.653   1.000
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
25         
 111 20000 9 0.7 0.5 2667337 141 99.3 0.1 
25         
 112 20000 14 0.7 0.7 2946845 272 99.3 0.1 
25         
 113 20000 15 0.7 0.8 2812597 116 99.3 0.0 
25         
 114 20000 10 0.5 0.5 3641724 132 99.5 0.0 
25         
 115 20000 10 0.7 0.5 2722547 209 99.3 0.1
Average ± SEM   0.685 ± 0.036 0.580 ± 0.060   0.000 ± 0.000 0.060 ± 0.011
Pairwise P   0.688   1.000
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
50         
 121 20000 16 0.6 0.8 3087510 106 99.4 0.0 
50         
 122 20000 9 0.6 0.5 3349505 156 99.4 0.0 
50         
 123 20000 5 0.9 0.3 2268593 69 99.1 0.0 
50         
 124 20000 12 0.7 0.6 2927000 84 99.3 0.0 
50         
 125 20000 10 0.7 0.5 2921240 79 99.3 0.0
Average ± SEM   0.699 ± 0.048 0.520 ± 0.090   0.000 ± 0.000 0.033 ± 0.004
Pairwise P   0.707   1.000
 

Blood

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
2006-03-14 24 hour Female Flow Cytometry Dermal Dermal x 91, 13 week
  Dose
(%)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 		Normochromatic Erythrocytes
Trend P:
No. Examined Total MN Cells Percent PCE MN Cells
per 1000		 No. Examined Total MN Cells Percent NCE MN Cells
per 1000
Untreated Control 
 0         
 71 20000 6 1.17 0.30 1684970 94 98.83 0.1 
 0         
 72 20000 13 0.93 0.65 2130449 113 99.07 0.1 
 0         
 73 20033 10 0.85 0.50 2343394 135 99.15 0.1 
 0         
 74 20000 14 0.86 0.70 2298716 308 99.14 0.1 
 0         
 75 20000 10 0.95 0.50 2086697 98 99.05 0
Average ± SEM   0.962 ± 0.060 0.530 ± 0.070   0.000 ± 0.000 0.069 ± 0.016
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
100         
 131 20000 11 0.7 0.6 2998158 412 99.3 0.1 
100         
 132 20000 11 0.9 0.6 2233798 107 99.1 0.0 
100         
 133 20000 8 1.2 0.4 1695001 186 98.8 0.1 
100         
 134 20000 9 2.6 0.5 764196 53 97.4 0.1 
100         
 135 20000 6 1.1 0.3 1878452 267 98.9 0.1
Average ± SEM   1.285 ± 0.344 0.450 ± 0.047   0.000 ± 0.000 0.101 ± 0.019
Pairwise P   0.677   0.116