Genetic Toxicology - Bacterial Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02303-0004-0000-4
Chemical Name:	Isobutene
CASRN:	115-11-7
NTP Study Type:	Genetic Toxicology - Bacterial Mutagenicity
NTP Study ID:	295184
Vehicle Control:	Air
Study Result:	Negative

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Table Instructions and Notes:
You are currently viewing the results for this Salmonella study's detailed data.
Individual strain data is presented as mean ± standard error.
Abbreviations are noted at bottom of page.
Trial summary calls are shown in parentheses.
Standard NTP Protocol

Strain: TA100
Dose	No Activation (Negative)		No Activation (Negative)		30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	110	7.5	143	14.3	141	10.4	127	4.7	142	11	131	2.2
0.001	98	1.9	152	5.3	147	12.5	121	8	131	12.9	132	13.5
0.002	100	4	151	11	124	9.1	114	8.6	142	1.9	128	11.6
0.007	101	4.9	135	2.6	134	4.3	117	7.2	146	5.5	130	3.2
0.013	81	1.9	124	11.9	95	.9	87	4.7	117	10.6	112	11.8
0.027	68	11.7	84	1.2	83	7.2	79	5.9	89	10.4	127	17.1
Positive Control	432	11.8	383	12.4	583	11.5	859	31.9	914	55.4	332	15.3

Strain: TA1535
Dose	No Activation (Negative)		No Activation (Negative)		30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	10	2.6	8	1.5	14	3.2	22	4.2	15	1.2	12	.9
0.001	11	1.2	10	1.2	20	2.7	15	2.1	11	1.5	13	1.3
0.002	15	2	14	2.3	20	3.9	16	.9	13	1.9	10	2
0.007	13	1	11	.6	14	3.7	16	2	12	3.2	11	1.3
0.013	10	1.8	14	.9	16	1.9	16	1.3	13	3	13	1.7
0.027	9	.9	13	2	13	2.9	16	2.2	13	2.1	13	3.1
Positive Control	359	14.5	340	8.8	121	11.6	129	5.7	164	9.6	63	4.8

Strain: TA97
Dose	No Activation (Negative)		No Activation (Negative)		30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	153	7.3	153	3.3	212	5	185	13	155	5.2	134	5.5
0.001	142	3.2	140	7.1	196	1.5	195	13.3	168	8.8	136	9
0.002	139	2.7	139	6.8	205	6.1	194	11.6	170	7.6	157	16.1
0.007	135	9.5	140	7.5	225	15.9	194	3	162	2.6	135	4.3
0.013	134	4.3	155	7.6	209	16.8	175	5.5	147	8	137	8
0.027	121	1.2	161	6.6	189	13.5	149	2	139	8.7	125	4.4
Positive Control	583	35.8	432	38.4	759	44.8	1505	74.3	942	19.7	2035	28.9

Strain: TA98
Dose	No Activation (Negative)		No Activation (Negative)		30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber		Plate Test - Gas Chamber
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	17	2.2	15	2.2	26	5.5	21	1.5	26	4.6	24	2.9
0.001	19	1.5	13	2.3	25	1.2	23	2.9	17	.6	19	1
0.002	17	1.5	14	.7	31	2.9	26	3.8	17	2.7	22	1.9
0.007	15	.7	14	.9	29	4.6	20	1.2	20	2	26	3.8
0.013	18	3.9	13	.6	19	4.1	19	2.7	27	3.6	19	2.4
0.027	11	1.5	10	1	15	1.5	14	1.5	23	.3	18	2.6
Positive Control	377	14.1	316	11.1	321	16.7	979	2	314	22.1	265	8

Abbreviations:
RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9
s = Slight Toxicity; p = Precipitate; x = Slight Toxicity and Precipitate; t = Toxic;