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Study Information
Study Attribute Value
CEBS Accession Number: 002-02945-0003-0000-5
Chemical Name: Trichloroethylene
CASRN: 79-01-6
NTP Study Type: Genetic Toxicology - Micronucleus
NTP Study ID: 679194
Strain and Species: B6C3F1 Mouse
Study Result: Male: Negative

Bone Marrow

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
1989-07-25 24 hour Male Slide Scoring Intraperitoneal Injection Intraperitoneal Injection x 3, 72 hour
  Dose
(mg/kg)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 0.835
No. Examined Total MN Cells Percent PCE MN Cells
per 1000
Vehicle Control Corn Oil 
 0         
 2172 2000 1 56.0 0.5 
 0         
 2187 2000 7 62.5 3.5 
 0         
 2190 2000 5 74.0 2.5 
 0         
 2203 2000 5 61.0 2.5 
 0         
 2209 2000 5 63.5 2.5
Average ± SEM   63.400 ± 2.950 2.300 ± 0.490
 
Test Chemical Trichloroethylene 
500         
 2188 2000 4 44.5 2.0 
500         
 2199 2000 5 68.5 2.5 
500         
 2208 2000 3 54.5 1.5 
500         
 2213 2000 9 60.5 4.5 
500         
 2217 2000 7 67.5 3.5
Average ± SEM   59.100 ± 4.450 2.800 ± 0.540
Pairwise P   0.242
 
Test Chemical Trichloroethylene 
1000         
 2164 2000 4 67.0 2.0 
1000         
 2169 2000 6 61.0 3.0 
1000         
 2195 2000 4 70.5 2.0 
1000         
 2211 2000 5 67.0 2.5 
1000         
 2218 2000 2 63.5 1.0
Average ± SEM   65.800 ± 1.630 2.100 ± 0.330
Pairwise P   0.619
 
Test Chemical Trichloroethylene 
2000         
 2162 2000 1 44.0 0.5 
2000         
 2166 2000 4 78.5 2.0 
2000         
 2170 2000 8 65.5 4.0 
2000         
 2194 2000 4 61.0 2.0 
2000         
 2200 2000 3 78.0 1.5 
2000         
 2205 2000 3 61.0 1.5
Average ± SEM   64.670 ± 5.240 1.920 ± 0.470
Pairwise P   0.732
 
Positive Control Dimethylbenzanthracene 
12.5       
 2173 2000 27 55.0 13.5 
12.5       
 2179 2000 18 59.0 9.0 
12.5       
 2192 2000 18 65.5 9.0 
12.5       
 2198 2000 11 52.5 5.5 
12.5       
 2212 2000 12 49.0 6.0
Average ± SEM   56.200 ± 2.840 8.600 ± 1.430
Pairwise P   Value less than 0.0001
 

Bone Marrow

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
1990-03-05 24 hour Male Slide Scoring Intraperitoneal Injection Intraperitoneal Injection x 3, 72 hour
  Dose
(mg/kg)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 0.028
No. Examined Total MN Cells Percent PCE MN Cells
per 1000
Vehicle Control Corn Oil 
 0         
 3001 2000 3 62.5 1.5 
 0         
 3045 2000 1 63.5 0.5 
 0         
 3054 2000 3 61.0 1.5 
 0         
 3069 2000 4 52.0 2.0 
 0         
 3072 2000 3 57.5 1.5
Average ± SEM   59.300 ± 2.090 1.400 ± 0.240
 
Test Chemical Trichloroethylene 
2000         
 3036 2000 2 63.0 1.0 
2000         
 3038 2000 6 65.5 3.0 
2000         
 3041 2000 5 59.5 2.5 
2000         
 3057 2000 5 70.0 2.5 
2000         
 3064 0 0 0 0.0
Average ± SEM   64.500 ± 2.210 2.250 ± 0.430
Pairwise P   0.089
 
Test Chemical Trichloroethylene 
2500         
 3006 2000 4 65.0 2.0 
2500         
 3013 2000 2 66.0 1.0 
2500         
 3017 0 0 0 0.0 
2500         
 3031 2000 4 64.5 2.0 
2500         
 3034 2000 5 58.0 2.5 
2500         
 3042 2000 11 65.5 5.5
Average ± SEM   63.800 ± 1.470 2.600 ± 0.760
Pairwise P   0.029
 
Test Chemical Trichloroethylene 
3000         
 3008 0 0 0.0 
3000         
 3010 0 0 0.0 
3000         
 3011 0 0 0.0 
3000         
 3015 0 0 0.0 
3000         
 3043 0 0 0.0 
3000         
 3058 0 0 0.0 
3000         
 3070 0 0 0.0
Average ± SEM   0.000 ± 0.000 0.000 ± 0.000
 
Positive Control Dimethylbenzanthracene 
12.5       
 3035 2000 9 60.0 4.5 
12.5       
 3037 2000 12 52.0 6.0 
12.5       
 3060 2000 3 50.0 1.5 
12.5       
 3065 2000 10 56.5 5.0 
12.5       
 3071 2000 10 65.5 5.0
Average ± SEM   56.800 ± 2.790 4.400 ± 0.760
Pairwise P   Value less than 0.0001
 

Blood

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
1989-07-25 24 hour Male Slide Scoring Intraperitoneal Injection Intraperitoneal Injection x 3, 72 hour
  Dose
(mg/kg)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 0.678
No. Examined Total MN Cells Percent PCE MN Cells
per 1000
Vehicle Control Corn Oil 
 0         
 2172 2000 3 3.4 1.5 
 0         
 2187 2000 10 3.9 5.0 
 0         
 2190 2000 5 3.6 2.5 
 0         
 2203 2000 8 3.0 4.0 
 0         
 2209 2000 7 3.3 3.5
Average ± SEM   3.440 ± 0.150 3.300 ± 0.600
 
Test Chemical Trichloroethylene 
500         
 2188 2000 8 3.8 4.0 
500         
 2199 2000 8 2.6 4.0 
500         
 2208 2000 3 3.1 1.5 
500         
 2213 2000 10 3.8 5.0 
500         
 2217 2000 5 3.5 2.5
Average ± SEM   3.360 ± 0.230 3.400 ± 0.620
Pairwise P   0.451
 
Test Chemical Trichloroethylene 
1000         
 2164 2000 6 3.4 3.0 
1000         
 2169 2000 7 3.2 3.5 
1000         
 2195 2000 8 3.3 4.0 
1000         
 2211 2000 5 5.2 2.5 
1000         
 2218 2000 6 3.7 3.0
Average ± SEM   3.760 ± 0.370 3.200 ± 0.250
Pairwise P   0.549
 
Test Chemical Trichloroethylene 
2000         
 2162 2000 8 4.1 4.0 
2000         
 2166 2000 5 2.7 2.5 
2000         
 2170 2000 4 3.6 2.0 
2000         
 2194 2000 7 3.1 3.5 
2000         
 2200 2000 6 2.4 3.0 
2000         
 2205 2000 0 0.0 0.0
Average ± SEM   3.180 ± 0.310 3.000 ± 0.350
Pairwise P   0.648
 
Positive Control Dimethylbenzanthracene 
12.5       
 2173 2000 15 2.2 7.5 
12.5       
 2179 2000 7 2.2 3.5 
12.5       
 2192 2000 24 1.2 12.0 
12.5       
 2198 2000 19 1.9 9.5 
12.5       
 2212 2000 25 2.4 12.5
Average ± SEM   1.980 ± 0.210 9.000 ± 1.640
Pairwise P   Value less than 0.0001
 

Blood

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
1990-03-05 24 hour Male Slide Scoring Intraperitoneal Injection Intraperitoneal Injection x 3, 72 hour
  Dose
(mg/kg)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 0.243
No. Examined Total MN Cells Percent PCE MN Cells
per 1000
Vehicle Control Corn Oil 
 0         
 3001 2000 5 3.1 2.5 
 0         
 3045 2000 5 3.1 2.5 
 0         
 3054 2000 4 3.3 2.0 
 0         
 3069 2000 5 5.0 2.5 
 0         
 3072 2000 5 4.3 2.5
Average ± SEM   3.760 ± 0.380 2.400 ± 0.100
 
Test Chemical Trichloroethylene 
2000         
 3036 2000 5 4.2 2.5 
2000         
 3038 2000 6 2.2 3.0 
2000         
 3041 2000 4 3.3 2.0 
2000         
 3057 2000 3 7.5 1.5 
2000         
 3064 0 0 0 0.0
Average ± SEM   4.300 ± 1.140 2.250 ± 0.320
Pairwise P   0.582
 
Test Chemical Trichloroethylene 
2500         
 3006 2000 6 2.4 3.0 
2500         
 3013 2000 5 2.4 2.5 
2500         
 3017 0 0 0 0.0 
2500         
 3031 2000 4 2.0 2.0 
2500         
 3034 2000 6 2.8 3.0 
2500         
 3042 2000 10 2.6 5.0
Average ± SEM   2.440 ± 0.130 3.100 ± 0.510
Pairwise P   0.172
 
Test Chemical Trichloroethylene 
3000         
 3008 0 0 0.0 
3000         
 3010 0 0 0.0 
3000         
 3011 0 0 0.0 
3000         
 3015 0 0 0.0 
3000         
 3043 0 0 0.0 
3000         
 3058 0 0 0.0 
3000         
 3070 0 0 0.0
Average ± SEM   0.000 ± 0.000 0.000 ± 0.000
 
Positive Control Dimethylbenzanthracene 
12.5       
 3035 2000 22 1.9 11.0 
12.5       
 3037 2000 26 2.6 13.0 
12.5       
 3060 2000 30 3.0 15.0 
12.5       
 3065 2000 14 2.3 7.0 
12.5       
 3071 2000 10 1.8 5.0
Average ± SEM   2.320 ± 0.220 10.200 ± 1.850
Pairwise P   Value less than 0.0001