Genetic Toxicology - Bacterial Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02997-0003-0000-2
Chemical Name:	Vat brown 1
CASRN:	2475-33-4
NTP Study Type:	Genetic Toxicology - Bacterial Mutagenicity
NTP Study ID:	977830
Vehicle Control:	Dimethyl Sulfoxide
Study Result:	Negative

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Table Instructions and Notes:
You are currently viewing the results for this Salmonella study's detailed data.
Individual strain data is presented as mean ± standard error.
Abbreviations are noted at bottom of page.
Trial summary calls are shown in parentheses.
Standard NTP Protocol

Strain: TA100
Dose	No Activation (Negative)		No Activation (Negative)		30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	106	9.6	141	8.1	101	2.6	84	4.6	127	7.9	139	10.7
100	119	7.5	138 p	9.7	113 p	10.7	97 p	7.3	127 p	4.5	110 p	7.5
333	94	.9	139 p	5.6	94 p	6.7	83 p	5.8	127 p	5.9	120 p	10.1
1000	107	6.1	127 p	4.7	73 p	6.5	114 p	4.9	107 p	6.2	117 p	6.1
3333	93 p	4.8	121 p	2.3	82 p	3.4	96 p	6.7	102 p	9.5	107 p	3.4
10000	76 p	5.8	112 p	4.1	73 p	6.1	81 p	7	95 p	5.9	104 p	9
Positive Control	354	13.7	398	22.4	567	21.5	564	47.5	461	40.2	652	21.5

Strain: TA1535
Dose	No Activation (Negative)		No Activation (Negative)		30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	14	1.3	21	1.2	13	1.2	14	2.3	13	1.2	11	2.7
100	19	3.4	22 p	1.5	11 p	2.3	9	0	10 p	1.2	12 p	.6
333	12	1.7	16 p	1.8	15 p	2.2	14	1.7	14 p	2.6	12 p	2
1000	11	1.5	13 p	1.5	14 p	1.5	7	1.2	10 p	1.9	11 p	1.2
3333	12 p	.9	16 p	1.2	11 p	3.2	12 p	1.9	13 p	2.7	10 p	.6
10000	16 p	1.2	18 p	2.7	10 p	2.4	14 p	1.8	15 p	2.5	15 p	2.3
Positive Control	455	23.6	371	6.3	97	3.3	443	32.7	95	7.3	142	16.1

Strain: TA97
Dose	No Activation (Equivocal)		No Activation (Negative)		30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	112	6.4	156	11.6	175	1.9	144	1.3	186	1.7	175	3.8
100	152	7.8	174 p	2.6	186 p	8.3	162	4.4	213 p	6.6	186 p	6.3
333	147	10.7	157 p	20	221 p	9.7	183	12.7	207 p	9.5	177 p	14.8
1000	154	3.2	158 p	9	202 p	3.2	176	10.9	193 p	6.2	182 p	10.5
3333	154 p	2.5	157 p	8.1	213 p	3.9	174 p	6.2	203 p	14.9	172 p	4.9
10000	154 p	8.1	140 p	6.1	120 x	31.9	108 p	3.8	169 p	10.2	152 p	7.9
Positive Control	627	12.7	442	35.9	363	17.8	355	6.7	357	6.9	422	18.8

Strain: TA98
Dose	No Activation (Negative)		No Activation (Negative)		30% RLI (Negative)		30% HLI (Negative)		10% RLI (Negative)		10% HLI (Negative)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM	Mean	±SEM
0	21	3.8	17	2.6	32	4.3	33	5.5	26	2.4	27	6.6
100	13	1.5	15 p	.3	39 p	1.8	41 p	4.7	26 p	3.5	35 p	1.5
333	16	2.6	15 p	1.3	45 p	.6	48 p	7.7	44 p	3.3	45 p	2.3
1000	12	1.8	11 p	1.5	53 p	.6	45 p	4.6	34 p	2.2	47 p	3.1
3333	14 p	.6	16 p	1.8	32 p	.9	50 p	2.9	16 p	3.2	29 p	3.5
10000	8 p	2.3	6 p	1	36 p	3	46 p	.9	19 p	3.8	21 p	1.2
Positive Control	507	31.8	561	23.3	164	3.7	514	18.2	323	2	530	10.3

Abbreviations:
RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9
s = Slight Toxicity; p = Precipitate; x = Slight Toxicity and Precipitate; t = Toxic;