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Study Information
Study Attribute Value
CEBS Accession Number: 002-03052-0002-0000-4
Chemical Name: bis(2-Chloroethyl)ether
CASRN: 111-44-4
NTP Study Type: Genetic Toxicology - Bacterial Mutagenicity
NTP Study ID: 579121
Vehicle Control: Dimethyl Sulfoxide
Study Result: Equivocal

Table Instructions and Notes:
You are currently viewing the results for this Salmonella study's detailed data.
Individual strain data is presented as mean ± standard error.
Abbreviations are noted at bottom of page.
Trial summary calls are shown in parentheses.
Standard NTP Protocol

Strain: TA100
Dose No Activation
(Equivocal) 		No Activation
(Negative) 		10% RLI
(Negative) 		10% RLI
(Negative) 		10% HLI
(Equivocal) 		10% HLI
(Equivocal) 		10% HLI
(Negative)
Protocol Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 122 8.3 119 7.9 152 14.5 157 13.8 127 13.3 109 2.6 176 9 
1         
 139 1.5 105 10.2 149 4.6 130 3.2 134 5.5 159 9 
3.3       
 142 5.7 95 11.3 132 2.4 140 5.4 140 6.7 145 4.7 
10         
 145 3.7 105 5.2 146 5.8 129 6.3 120 3.2 146 3.4 
16         
 105 13.6 
33         
 141 7.5 110 4 157 7.7 142 6.4 136 14.1 140 6.2 162 2.9 
67         
 134 8.4 
100         
 145 7.9 116 11.5 156 11.5 154 5.2 169 5.5 110 9.6 167 15.9 
167         
 107 4.1
Positive Control 1518 31.3 1309 25.5 3012 84.3 2333 153 2232 60 2677 145 2615 100.1

Strain: TA1535
Dose No Activation
(Negative) 		No Activation
(Negative) 		10% RLI
(Negative) 		10% RLI
(Negative) 		10% RLI
(Positive) 		10% HLI
(Equivocal) 		10% HLI
(Equivocal) 		10% HLI
(Negative) 		10% HLI
(Negative) 		10% HLI
(Negative)
Protocol Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 13 2.3 15 1.2 8 2.5 15 4 8 2.7 12 3.5 8 1.7 5 1.9 15 3.8 26 1.5 
1         
 14 2.1 13 1.2 12 1.7 7 2.3 15 3 12 1.3 10 1.5 25 4.2 
3.3       
 18 2.3 14 2.1 12 2.2 14 3.5 13 1 17 2.6 11 1.5 26 3.3 
10         
 15 4 13 .3 9 1.7 12 1.5 14 1.8 15 2.1 18 1.9 5 1.2 10 .7 26 .3 
16         
 9 3.8 13 .9 
33         
 18 1.2 8 .7 8 1.7 13 3.5 25 2.7 18 1.2 17 2.7 9 .9 12 .9 29 4.1 
67         
 13 .9 8 1.5 
100         
 10 9.7 10 1.5 9 .9 15 2.2 40 5.2 39 8.5 11 2.4 7 .6 13 1.2 35 6.5
Positive Control 1025 108.6 900 48 77 39.2 249 18.4 360 11.9 348 19.7 296 15.6 87 15.4 339 24.1 311 44.5

Strain: TA1537
Dose No Activation
(Negative) 		No Activation
(Negative) 		10% RLI
(Negative) 		10% RLI
(Negative) 		10% HLI
(Negative) 		10% HLI
(Negative)
Protocol Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 4 1.2 11 1 9 .3 18 2.2 8 1.3 22 2.6 
1         
 9 .7 12 1.2 4 .6 17 .6 7 .7 19 1.8 
3.3       
 5 .7 15 1.9 10 .9 17 0 10 1.5 15 1.5 
10         
 4 .6 8 .9 12 1.2 16 .6 13 1.7 22 4.2 
33         
 7 1.8 8 1.2 11 .6 7 .9 10 3.2 12 .9 
100         
 t 11 2 8 3.5 16 2.8 8 .3 13 1.5
Positive Control 1341 167.9 297 53 55 14.2 268 23 43 3 171 20.5

Strain: TA98
Dose No Activation
(Equivocal) 		No Activation
(Negative) 		10% RLI
(Negative) 		10% RLI
(Negative) 		10% RLI
(Negative) 		10% RLI
(Positive) 		10% HLI
(Equivocal) 		10% HLI
(Negative)
Protocol Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation Preincubation
ug/Plate Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM Mean ±SEM 
 0         
 23 2.8 26 6 33 3.3 37 1.9 15 1.2 8 1.5 26 1.7 24 2 
1         
 29 3.2 23 2.3 31 1.5 29 4.9 12 1.7 34 4 35 3.2 30 6.9 
3.3       
 41 2.6 22 3.8 30 3.5 40 6.6 11 2.3 49 4.8 39 4.1 36 1.2 
10         
 31 1.5 28 2.2 26 3.3 44 1 14 .6 51 3.5 37 7 30 4.1 
33         
 36 6.1 17 2 31 7.2 49 4.5 14 .9 43 2.3 26 2.5 27 1 
100         
 26 1.5 20 2 28 2.5 36 1.3 10 3.5 44 5.6 32 7.1 28 1.5
Positive Control 679 34.7 181 12.5 1614 67 521 3.5 1036 63.2 555 35.2 1411 48.2 2292 138.7

Abbreviations:
RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9
s = Slight Toxicity; p = Precipitate; x = Slight Toxicity and Precipitate; t = Toxic;