Parent Title:
Center for Integrative Research on Childhood Leukemia and the Environment (CIRCLE)
Grant Number:
Principal Investigator:
Buffler, Patricia (PI of Parent Grant CIRCLE); Rappaport, Stephen (Leader of Project 2)
Institution:
University of California, Berkeley
Most Recent Award Year:
2009
Lifestage of Participants:
Exposure:
Prenatal; Infant (0-1 year); Youth (1-14 years)
Assessment:
Infant (0-1 year); Youth (1-14 years)
Exposures:
Air Pollutants:
Polycyclic aromatic hydrocarbons (PAHs); Tobacco smoke
Brominated Compounds:
Polybrominated diphenyl ethers (PBDEs)
Chlorinated Compounds:
Polychlorinated biphenyls (PCBs)
Health Outcomes:
Cancer Outcomes:
Childhood leukemia
Biological Sample:
Blood; Newborn dried blood spots
Environmental Sample:
Dust
Abstract:
Previous case-control studies of childhood leukemia mostly relied on self-reported exposure which lack specificity and may suffer from recall bias. The proposed study builds upon a large case-control study, the Northern California Childhood Leukemia Study (NCCLS) to improve chemical exposure assessment, using available home dust samples and a variety of biospecimens. Preliminary NCCLS findings suggest that house dust can provide useful quantitative surrogates for in-home exposures to toxic contaminants. In order to assess whether persistent contaminants such as nicotine (a surrogate for ETS), polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (PBDEs) accumulate in house dust over several years, the proposed study will obtain an additional household-dust sample from each of 150 homes in the NCCLS population for which we have existing dust samples. To further validate the use of house dust as a measure of children's exposures to toxic substances, we will measure nicotine, PCBs, and PBDEs in serum samples obtained from about 250 childhood leukemia cases at diagnosis and then determine correlations between analyte levels in serum and house dust. In addition, the NCCLS obtained archived newborn dried blood spot (DBS) collected at birth. Since blood and DBS contain adducts of potential carcinogens with hemoglobin (Hb) and human serum albumin (HSA), they offer opportunities for quantifying children's exposures and internal doses during one or two months prior to collection. Using methods developed in our laboratory, we will detect and profile cysteinyl adducts of HSA in pre-treatment diagnostic blood from children with leukemia and in newborn DBS from a subset of 200 children (100 cases and 100 controls; these subjects will be among those investigated for DNA methylation patterns in Project 3). By comparing DBS-adduct profiles between childhood leukemia case and control children, we will detect particular adducts that are associated with disease status. Then, after chemically identifying these adducts and their likely precursors, we will pinpoint early life exposures that increase the risk of childhood leukemia, and possible changes in levels of important adducts between birth and diagnosis.
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Related NIEHS-Funded Study Populations
California Childhood Leukemia Study (CCLS)
Principal Investigator:
Metayer, Catherine (Buffler, Patricia)
| Study Population Page Study Population c79
Institution:
University of California, Berkeley
Location:
California
Number of Participants::
3,523 (2,213 cases and 1,310 controls)
Brief Description::
This is a population-based case-control study to examine the relationship between environmental exposures, genetic factors, and childhood leukemia. The study has undergone multiple phases of enrollment since 1995 and and now includes over 2,000 leukemia cases.